Gene Expression Data
Assay Details
Assay
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Reference:
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J:214586
Duan X, et al., Type II cadherins guide assembly of a direction-selective retinal circuit. Cell. 2014 Aug 14;158(4):793-807
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5698645
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Gene symbol:
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Neto1
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Gene name:
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neuropilin (NRP) and tolloid (TLL)-like 1
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Reporter:
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Cre
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Antibody:
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anti-cre
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Detection system:
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Secondary antibody coupled to DyLight 649
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Results
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Specimen
1C:
postnatal; Neto1tm1.1(cre)Jrs/Neto1 +
(more )
Note: Triple labeled: red - Neto1 reporter; green - Rcvrn (assay MGI:5699334); blue - NeuroTrace Nissl (nuclei).
Specimen
1C:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Neto1tm1.1(cre)Jrs/Neto1+
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Neto1 reporter; green - Rcvrn (assay MGI:5699334); blue - NeuroTrace Nissl (nuclei).
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Structure |
Level |
Pattern |
Image |
Note |
TS28: retina inner nuclear layer |
Present |
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1C left
,
1C center
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Expression present in type 5 bipolar cells; their soma are in the inner nuclear layer and they arborize in the inner plexiform layer.
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Specimen
3A:
postnatal; Cdh8tm1Mta/Cdh8 +, Neto1tm1.1(cre)Jrs/Neto1 +
(more )
Note: Triple labeled: red - Neto1 reporter; green - Cdh8 reporter (assay MGI:5699341); blue - NeuroTrace Nissl (nuclei).
Specimen
3A:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Cdh8tm1Mta/Cdh8+ Neto1tm1.1(cre)Jrs/Neto1+
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Neto1 reporter; green - Cdh8 reporter (assay MGI:5699341); blue - NeuroTrace Nissl (nuclei).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: retina inner nuclear layer |
Present |
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3A right
,
3A left
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Expression present in type 5 bipolar cells; their soma are in the inner nuclear layer and they arborize in the inner plexiform layer.
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