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Gene Expression Data
Assay Details
Assay
Reference: J:213921 Yang L, et al., Hypertrophic chondrocytes can become osteoblasts and osteocytes in endochondral bone formation. Proc Natl Acad Sci U S A. 2014 Aug 19;111(33):12097-102
Assay type: Recombinase reporter
MGI Accession ID: MGI:5697720
Gene symbol: Col10a1
Gene name: collagen, type X, alpha 1
Reporter: lacZ
Probe: lacZ probe3
Assay notes: Induction was achieved by treatment with tamoxifen dissolved stepwise in ethanol and corn oil to a final concentration of 10 mg/mL at a dosage of 0.1 mg/g body weight at time points indicated.
Results
Specimen 3B E18.5 Osx: embryonic day 18.5; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated at E13.5, Double labeled: cyan - recombinase reporter; brown - Sp7.
Structure Level Pattern Image Note
TS26: tibia Present 3B E18.5 Osx Recombinase reporter staining is detected in some Sp7 expressing preosteoblasts.
Specimen 3B E18.5 Col1a1: embryonic day 18.5; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated at E13.5, Double labeled: cyan - recombinase reporter; brown - Col1a1.
Structure Level Pattern Image Note
TS26: tibia Present 3B E18.5 Col1a1 Recombinase reporter staining is detected in some Col1a1 expressing osteoblasts.
Specimen 3B E18.5 Collagen I: embryonic day 18.5; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated at E13.5, Double labeled: cyan - recombinase reporter; brown - Collagen I.
Structure Level Pattern Image Note
TS26: tibia Present 3B E18.5 Collagen I Recombinase reporter staining is detected in some cells expressing Collagen type I.
Specimen 3B P5 Col1a1: postnatal day 5; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated at E13.5, Double labeled: cyan - recombinase reporter; brown - Col1a1.
Structure Level Pattern Image Note
TS28: tibia Present 3B P5 Col1a1 Recombinase reporter staining is detected in some Col1a1 expressing osteoblasts.
Specimen 3B P10 Sost: postnatal day 10; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated at E13.5, Double labeled: cyan - recombinase reporter; brown - Sost.
Structure Level Pattern Image Note
TS28: tibia Present 3B P10 Sost Recombinase reporter staining is detected in Sost expressing osteocytes.
Specimen S6 Tibia: embryonic day 15.5; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated at E14.5. Double labeled: blue - recombinase reporter; black - Col1a1.
Structure Level Pattern Image Note
TS23: tibia Present S6Tibia left/right However, recombinase reporter staining does not overlap with Col1a1 expression.
Specimen S6 Humerus: embryonic day 15.5; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated at E14.5. Double labeled: blue - recombinase reporter; black - Col1a1.
Structure Level Pattern Image Note
TS23: humerus Present S6Humerus left/right However, recombinase reporter staining does not overlap with Col1a1 expression.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory