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Gene Expression Data
Assay Details
Assay
Reference: J:213921 Yang L, et al., Hypertrophic chondrocytes can become osteoblasts and osteocytes in endochondral bone formation. Proc Natl Acad Sci U S A. 2014 Aug 19;111(33):12097-102
Assay type: Recombinase reporter
MGI Accession ID: MGI:5697665
Gene symbol: Col10a1
Gene name: collagen, type X, alpha 1
Reporter: lacZ
Direct Detection
Assay notes: Induction was achieved by treatment with tamoxifen dissolved stepwise in ethanol and corn oil to a final concentration of 10 mg/mL at a dosage of 0.1 mg/g body weight at time points indicated. Without induction, no cre activity is detected.
Results
Specimen 2C Hindlimb: embryonic day 15.5; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated at E12.5.
Structure Level Pattern Image Note
TS23: femur Present 2C hindlimb Recombinase reporter staining is detected in the hypertrophic zones.
TS23: fibula Present 2C hindlimb Recombinase reporter staining is detected in the hypertrophic zones.
TS23: tibia Present 2C hindlimb Recombinase reporter staining is detected in the hypertrophic zones.
Specimen 2C Forelimb: embryonic day 15.5; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated at E12.5.
Structure Level Pattern Image Note
TS23: humerus Present 2C forelimb Recombinase reporter staining is detected in the hypertrophic zones.
TS23: metacarpal bone Present 2C forelimb Recombinase reporter staining is detected in the hypertrophic zones.
TS23: radius Present 2C forelimb Recombinase reporter staining is detected in the hypertrophic zones.
TS23: scapula Present 2C forelimb Recombinase reporter staining is detected in the hypertrophic zones.
TS23: ulna Present 2C forelimb Recombinase reporter staining is detected in the hypertrophic zones.
Specimen 2D Tibia E14.5: embryonic day 14.5; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated at E12.5.
Structure Level Pattern Image Note
TS22: tibia cartilage condensation Absent 2D E14.5 Tibia
Specimen 2D Tibia E15.5: embryonic day 15.5; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated at E12.5.
Structure Level Pattern Image Note
TS23: tibia Present 2D E15.5 Tibia Recombinase reporter staining is detected in the hypertrophic zone although in a smaller population than in the humerus.
Specimen 2D Tibia E16.5/inset: embryonic day 16.5; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated at E12.5.
Structure Level Pattern Image Note
TS24: tibia Present 2D E16.5 Tibia/insert Recombinase reporter staining is detected in primary ossification centers.
Specimen 2D Humerus E14.5: embryonic day 14.5; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated at E12.5.
Structure Level Pattern Image Note
TS22: humerus cartilage condensation Absent 2D E14.5 Humerus
Specimen 2D Humerus E15.5: embryonic day 15.5; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated at E12.5.
Structure Level Pattern Image Note
TS23: humerus Present 2D E15.5 Humerus Recombinase reporter staining is detected in the hypertrophic zone in a larger population than in the tibia.
Specimen 2D Humerus E16.5/inset: embryonic day 16.5; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated at E12.5.
Structure Level Pattern Image Note
TS24: humerus Present 2D E16.5 Humerus/insert Recombinase reporter staining is detected in fewer hypertrophic chondrocytes in teh hypertrophic zone but are present in many cells of the primary ossification centers.
Specimen 3A E15.5: embryonic day 15.5; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated at E13.5.
Structure Level Pattern Image Note
TS23: tibia Present 3A E15.5 Recombinase reporter staining is detected in hypertrophic chondrocytes.
Specimen 3A E18.5: embryonic day 18.5; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated at E13.5.
Structure Level Pattern Image Note
TS26: tibia Present 3A E18.5 Recombinase reporter expression is detected in primary ossification centers but not hypertrophic chondrocytes.
Specimen 3A P5: postnatal day 5; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated at E13.5.
Structure Level Pattern Image Note
TS28: long bone diaphysis Present 3A P5/insets Recombinase reporter staining is detected in the diaphysis and trabecular region but not the hypertrophic chondrocytes.
TS28: tibia Present 3A P5/insets Recombinase reporter staining is detected in the diaphysis and trabecular region but not the hypertrophic chondrocytes.
Specimen 3A P1m: postnatal month 1; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated at E13.5.
Structure Level Pattern Image Note
TS28: long bone diaphysis Present 3A P1m/insets Recombinase reporter staining is detected in the diaphysis and trabecular region but not the hypertrophic chondrocytes.
TS28: tibia Present 3A P1m/insets Recombinase reporter staining is detected in the diaphysis and trabecular region but not the hypertrophic chondrocytes.
Specimen 2E Fibula: embryonic day 15.5; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated at E14.5.
Structure Level Pattern Image Note
TS23: fibula Present 2E Fibula Recombinase reporter staining is detected in hypertrophic chondrocytes.
Specimen 2E Tibia: embryonic day 15.5; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated at E14.5.
Structure Level Pattern Image Note
TS23: tibia Present 2E Tibia Recombinase reporter staining is detected in hypertrophic chondrocytes.
Specimen 2E Femur: embryonic day 15.5; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated at E14.5.
Structure Level Pattern Image Note
TS23: femur Present 2E Femur Recombinase reporter staining is detected in hypertrophic chondrocytes and a few cells in the forming primary ossification centers.
Specimen 2E Radius: embryonic day 15.5; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated at E14.5.
Structure Level Pattern Image Note
TS23: radius Present 2E Radius Recombinase reporter staining is detected in hypertrophic chondrocytes and more cells in the forming primary ossification centers.
Specimen 2E Humerus: embryonic day 15.5; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated at E14.5.
Structure Level Pattern Image Note
TS23: humerus Present 2E Humerus Recombinase reporter staining is detected in hypertrophic chondrocytes and many cells in the forming primary ossification centers.
Specimen 2F Col1a1 Fibula: embryonic day 16.0; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated E14.5. Double labeled: blue - reporter recombinase; black - Col1a1.
Structure Level Pattern Image Note
TS24: fibula Present 2F Fibula top/bottom However, there is no overlap between recombinase reporter staining and Col1a1 transcript expression.
Specimen 2F Col1a1 Tibia: embryonic day 16.0; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated E14.5. Double labeled: blue - reporter recombinase; black - Col1a1.
Structure Level Pattern Image Note
TS24: tibia Present 2F Tibia top/bottom Recombinase reporter staining overlaps with Col1a1 transcript expression in few cells.
Specimen 2F Col1a1 Femur: embryonic day 16.0; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated E14.5. Double labeled: blue - reporter recombinase; black - Col1a1.
Structure Level Pattern Image Note
TS24: femur Present 2F Femur top/bottom Recombinase reporter staining overlaps with Col1a1 transcript expression in some cells.
Specimen 2F Col1a1 Humerus: embryonic day 16.0; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated E14.5. Double labeled: blue - reporter recombinase; black - Col1a1.
Structure Level Pattern Image Note
TS24: humerus Present 2F Humerus Col1a1 top/bottom Recombinase reporter staining overlaps with Col1a1 transcript expression in several cells.
Specimen 2F Osx Humerus: embryonic day 16.0; Col10a1tm3.1(cre/ERT)Ksec/Col10a1+, Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Tamoxifen treated E14.5. Double labeled: blue - reporter recombinase; brown - Sp7.
Structure Level Pattern Image Note
TS24: humerus Present 2F Humerus OSX top/bottom Recombinase reporter staining overlaps with Sp7 transcript expression in several cells.
Specimen S8A E16.5: embryonic day 16.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Col10a1tm2.1(cre)Ksec/Col10a1+ (more )
Note: Conditional mutant. Tamoxifen treated at E13.5.
Structure Level Pattern Image Note
TS24: tibia Present S8A E16.5 Recombinase reporter staining is detected in the primary ossification centers with HC-derived cells present in the trabecular and cortical bones, including the endosteum region.
Specimen S8A E17.5: embryonic day 17.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+, Col10a1tm2.1(cre)Ksec/Col10a1+ (more )
Note: Conditional mutant. Tamoxifen treated at E13.5.
Structure Level Pattern Image Note
TS25: tibia Present S8A E17.5 Recombinase reporter staining is detected in the primary ossification centers with HC-derived cells present in the trabecular and cortical bones, including the endosteum region.
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03/18/2025
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