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Gene Expression Data
Assay Details
Assay
Reference: J:79513 Hayashi K, et al., SMAD1 signaling is critical for initial commitment of germ cell lineage from mouse epiblast. Mech Dev. 2002 Oct;118(1-2):99
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5696353
Gene symbol: Smad9
Gene name: SMAD family member 9
Antibody: anti-PS-1
Assay notes: The antibody used in this assay recognizes the phosphorylated form of the protein translated from three genes. RT-PCR experiments presented in this paper suggest that all 3 genes are transcribed at this time, so all three family members have been annotated when appropriate.
Results
Specimen 5A: embryonic day 5.5 (more )
Note: pre-gastrula stage
Structure Level Pattern Image Note
TS8: epiblast Present Regionally restricted 5A Expression is seen in the proximal region.
TS8: primitive endoderm Present 5A Expression is seen in the visceral endoderm.
TS8: extraembryonic ectoderm Absent 5A
Specimen 5B: embryonic day 5.5 (more )
Note: pre-gastrula stage. Embedded in methacrylate.
Structure Level Pattern Image Note
TS8: epiblast Present Regionally restricted 5B Expression is seen in the proximal region.
TS8: primitive endoderm Present 5B Expression is seen in the visceral endoderm.
TS8: extraembryonic ectoderm Absent 5B
Specimen 5C: embryonic day 5.75 (more )
Note: early streak stage
Structure Level Pattern Image Note
TS9: embryo Present Regionally restricted 5C Expression is seen in the proximal region of the epiblast.
TS9: primitive endoderm Absent 5C No expression is seen in the visceral endoderm.
TS9: extraembryonic ectoderm Absent 5C
Specimen 5D: embryonic day 5.75 (more )
Note: early streak stage. Embedded in methacrylate.
Structure Level Pattern Image Note
TS9: embryo Present Regionally restricted 5D Expression is seen in the proximal region of the epiblast.
TS9: primitive endoderm Absent 5D No expression is seen in the visceral endoderm.
TS9: extraembryonic ectoderm Absent 5D
Specimen 5E: embryonic day 6.0 (more )
Note: mid-streak stage
Structure Level Pattern Image Note
TS10: embryo Present Regionally restricted 5E Expression is seen in the proximal region of the epiblast.
TS10: primitive endoderm Absent 5E No expression is seen in the visceral endoderm.
TS10: extraembryonic ectoderm Absent 5E
Specimen 5F: embryonic day 6.0 (more )
Note: mid-streak stage. Embedded in methacrylate.
Structure Level Pattern Image Note
TS10: embryo Present Regionally restricted 5F Expression is seen in the proximal region of the epiblast.
TS10: primitive endoderm Absent 5F No expression is seen in the visceral endoderm.
TS10: extraembryonic ectoderm Absent 5F
Specimen 7D: embryonic day 6.25 (more )
Structure Level Pattern Image Note
TS10: embryo Present Regionally restricted 7D Expression is localized in the proximal region of the epiblast.
Specimen 7E: embryonic day 6.25; Smad1tm1Tako/Smad1tm1Tako (more )
Structure Level Pattern Image Note
TS10: embryo Present Regionally restricted 7E As in wild type, expression is localized in the proximal region of the epiblast. However, the amount of phosphorylated protein is less.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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