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Gene Expression Data
Assay Details
Assay
Reference: J:85957 Bekku Y, et al., Molecular cloning of Bral2, a novel brain-specific link protein, and immunohistochemical colocalization with brevican in perineuronal nets. Mol Cell Neurosci. 2003 Sep;24(1):148-59
Assay type: RNA in situ
MGI Accession ID: MGI:5695208
Gene symbol: Hapln4
Gene name: hyaluronan and proteoglycan link protein 4
Probe: Bral2 cDNA1
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: Two probes were used to study the expression of this gene; it is possible MGI:5694792 was used to generate some or all of these data.
Results
Specimen 3C/E/F: postnatal adult (more )
Note: sagittal section
Structure Level Pattern Image Note
TS28: cerebellar cortex Present 3C Expression appears to be specific for neurons.
TS28: inferior colliculus Present 3C Expression appears to be specific for neurons.
TS28: deep cerebellar nucleus Present 3C Expression appears to be specific for neurons.
TS28: cerebellum Purkinje cell layer Present 3E , 3C
TS28: cerebellum granule cell layer Present Single cells 3C , 3E Expression in Golgi cells.
TS28: cerebellum molecular layer Present Single cells 3C , 3F Expression in stellate and basket cells.
Specimen 3H: postnatal adult (more )
Note: sagittal section; double labeled: blue - Hapln4; brown - Nefh (assay MGI:5695224).
Structure Level Pattern Image Note
TS28: cortical layer III Present 3H Expression is prominent in this layer.
TS28: cortical layer V Present 3H Expression is prominent in this layer.
TS28: cortical layer II Present 3H Expression spans layers II-VI.
TS28: cortical layer IV Present 3H Expression spans layers II-VI.
TS28: cortical layer VI Present 3H Expression spans layers II-VI.
Specimen 3I: postnatal adult (more )
Note: coronal section
Structure Level Pattern Image Note
TS28: trigeminal nucleus Present 3I
Specimen 3J: postnatal adult (more )
Note: coronal section
Structure Level Pattern Image Note
TS28: pontine reticular nucleus Present 3J
Specimen 3K: postnatal adult (more )
Note: coronal section; double labeled: blue - Hapln4 RNA; brown - Hapln4 protein (assay MGI:5695223).
Structure Level Pattern Image Note
TS28: medial geniculate nucleus Trace 3K More protein is detected than mRNA.
Specimen not shown: postnatal adult (more )
Structure Level Pattern Image Note
TS28: CA3 pyramidal cell layer Present
TS28: cochlear nucleus Present
TS28: oculomotor nucleus Present
TS28: substantia nigra Present
TS28: subthalamic nucleus Present
TS28: superior olivary nucleus Present
TS28: vestibular nucleus Present
TS28: nucleus of trapezoid body Present Expression in the trapezoid body.
TS28: ventral posterior medial thalamic nucleus Trace More protein is detected than mRNA.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory