Gene Expression Data
Assay Details
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Assay
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Reference:
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J:36520
Wakisaka S, et al., Protein gene-product 9.5 in developing mouse circumvallate papilla: comparison with neuron-specific enolase and calcitonin gene-related peptide. Anat Embryol (Berl). 1996 Oct;194(4):365-72
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5694659
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Gene symbol:
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Calca
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Gene name:
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calcitonin/calcitonin-related polypeptide, alpha
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Antibody:
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anti-CGRP
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
2E:
postnatal adult
(more  )
Specimen
2E:
(close  )
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Genetic Background:
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ICR
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: circumvallate papillae |
Present |
Regionally restricted |
2E
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Expression in thin nerve fibers forming the subgemmal nerve plexus in the lamina propria, some penetrating the taste buds. No expression in neuronal perikarya.
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| TS28: taste bud |
Absent |
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2E
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No expression in taste bud cellular elements.
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Specimen
3C:
embryonic day 12.5
(more )
Specimen
3C:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: tongue epithelium |
Weak |
Regionally restricted |
3C
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Weak expression in a small number of nerve fibers in the lamina propria just beneath the apical epithelium.
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Specimen
not shown E13:
embryonic day 13.5
(more )
Specimen
not shown E13:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
not shown E14:
embryonic day 14.5
(more )
Specimen
not shown E14:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
not shown E15:
embryonic day 15.5
(more )
Specimen
not shown E15:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: circumvallate papillae |
Present |
Regionally restricted |
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Expression in an increased number of nerve fibers in the lamina propria, some of these penetrating the apical epithelium.
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Specimen
5B:
embryonic day 17.5
(more )
Specimen
5B:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: circumvallate papillae |
Present |
Regionally restricted |
5B
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Expression in a small number of nerve fibers penetrating the apical epithelium, at the border of outer lateral trench wall and lingual epithelium, and the inner lateral epithelium of the trench.
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Specimen
not shown E18:
embryonic day 18.5
(more )
Specimen
not shown E18:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
not shown P0:
postnatal day 0
(more )
Specimen
not shown P0:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: circumvallate papillae |
Present |
Regionally restricted |
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Authors report temporal changes in number of nerve fibers were almost identical to that seen for expression of PGP- 9.5 (see assay MGI:5694656).
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Specimen
not shown P5:
postnatal day 5
(more )
Specimen
not shown P5:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: circumvallate papillae |
Present |
Regionally restricted |
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Expression in round- or oval-shaped cell in inner trench wall epithelium, laterac changing to a spindle-like appearance. Expression in apical epithelium disappeared.
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Specimen
9B:
postnatal day 14
(more )
Specimen
9B:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal day 14
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: circumvallate papillae |
Present |
Regionally restricted |
9B
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Authors state that intensity and distribution of expression in nerve fibers was almost identical to that seen in adults (compare with Figure 2E)
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Analysis Tools