Gene Expression Data
Assay Details
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Assay
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Reference:
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J:84432
Qi Y, et al., Gli2 is required for normal Shh signaling and oligodendrocyte development in the spinal cord. Mol Cell Neurosci. 2003 Jul;23(3):440-50
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5694633
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Gene symbol:
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Pdgfra
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Gene name:
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platelet derived growth factor receptor, alpha polypeptide
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Probe:
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Pdgfra probe16
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
4A:
embryonic day 12.5
(more  )
Specimen
4A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord ventricular layer |
Present |
Regionally restricted |
4A
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Expression was detected in the ventral ventricular zone.
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| TS20: spinal cord |
Present |
Regionally restricted |
4A
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Expression was noted in a few migratory oligodendrocyte progenitor cells.
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Specimen
4B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4C:
embryonic day 13.5
(more )
Specimen
4C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: spinal cord |
Present |
Regionally restricted |
4D
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Expression was detected in the ventral spinal cord.
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Specimen
4E:
embryonic day 14.5
(more )
Specimen
4E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord |
Present |
Widespread |
4E
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Expression was detected throughout the entire spinal cord.
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Specimen
4F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord |
Present |
Regionally restricted |
4F
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Expression was restricted to the ventral half of the mutant spinal cord.
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Specimen
4G:
embryonic day 16.5
(more )
Specimen
4G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: spinal cord |
Present |
Regionally restricted |
4G
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Expression was detected in oligodendrocyte progenitor cells.
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Specimen
4H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: spinal cord |
Present |
Regionally restricted |
4H
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Expression was detected in oligodendrocyte progenitor cells. There was only a slight decrease in the number of cells detected in the mutant spinal cord.
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Specimen
4I:
embryonic day 18.5
(more )
Specimen
4I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: spinal cord |
Present |
Regionally restricted |
4I
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Expression was detected in oligodendrocyte progenitor cells.
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Specimen
4J:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: spinal cord |
Present |
Regionally restricted |
4J
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Expression was detected in oligodendrocyte progenitor cells in similar numbers compared to wild type.
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Specimen
8E:
embryonic day 14.5
(more )
Specimen
8E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: corpus striatum |
Present |
Regionally restricted |
8E
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Expression was detected in the oligodendrocyte progenitor cells.
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| TS22: neocortex |
Present |
Regionally restricted |
8E
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Expression was detected in the oligodendrocyte progenitor cells.
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| TS22: telencephalon septum |
Present |
Regionally restricted |
8E
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Expression was detected in the oligodendrocyte progenitor cells.
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Specimen
8F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: corpus striatum |
Present |
Regionally restricted |
8F
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As in wild type, expression was detected in the oligodendrocyte progenitor cells in the mutant.
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| TS22: neocortex |
Present |
Regionally restricted |
8F
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As in wild type, expression was detected in the oligodendrocyte progenitor cells in the mutant.
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| TS22: telencephalon septum |
Present |
Regionally restricted |
8F
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As in wild type, expression was detected in the oligodendrocyte progenitor cells in the mutant.
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Analysis Tools