Probe:	Olig2 probe12
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	1B	Expression was detected in ventral neuroepithelium.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	1E	Expression in the motor neuron domain was not significantly decreased in the mutant.

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Present	Regionally restricted	1H	Expression was detected in the ventral ventricular zone.
TS20: spinal cord	Present	Regionally restricted	1H	Expression was detected in the ventral spinal neuroepithelium.

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Ambiguous	Regionally restricted	1K	Expression in the ventral ventricular zone was not up-regulated in the mutant.
TS20: spinal cord	Present	Regionally restricted	1K	Expression was detected in the ventral spinal neuroepithelium.

Structure	Level	Pattern	Image	Note
TS16: future spinal cord	Present	Regionally restricted	2A	Expression was detected in ventral neuroepithelium.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Weak	Regionally restricted	2B	Expression was downregulated in the ventral neuroepithelium at E11.5.

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Present	Regionally restricted	2C	Expression was detected in the ventral ventricular zone.
TS20: spinal cord	Present	Regionally restricted	2C	Expression was detected in the ventral neuroepithelium.

Structure	Level	Pattern	Image	Note
TS16: future spinal cord	Present	Regionally restricted	2E	Expression in the motor neuron domain was not significantly decreased in the mutant.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Weak	Regionally restricted	2F	Expression was downregulated in the ventral neuroepithelium at E11.5 as in wild type.

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Ambiguous	Regionally restricted	2G	Expression in the ventral ventricular zone was not up-regulated in the mutant.
TS20: spinal cord	Present	Regionally restricted	2G	Expression was detected in the ventral neuroepithelium.

Structure	Level	Pattern	Image	Note
TS21: spinal cord ventricular layer	Strong		3C
TS21: spinal cord	Present	Regionally restricted	3C	Expression was detected in oligodendrocyte pregenitor cells that have migrated out of the ventricular zone and into the surrounding regions.

Structure	Level	Pattern	Image	Note
TS21: spinal cord ventricular layer	Trace		3D
TS21: spinal cord	Present	Regionally restricted	3D	Few progenitor cells are generated from the reduced neuroepithelium and migrate into the ventral gray matter region.

Structure	Level	Pattern	Image	Note
TS22: spinal cord ventricular layer	Strong		3E
TS22: spinal cord	Present	Regionally restricted	3E	Expression was detected in oligodendrocyte pregenitor cells.

Structure	Level	Pattern	Image	Note
TS22: spinal cord ventricular layer	Absent		3F
TS22: spinal cord	Present	Regionally restricted	3F	The number of oligodendrocyte pregenitor cells is still lower in the mutant compared to wild type, especially at the peripheral region.

Structure	Level	Pattern	Image	Note
TS24: spinal cord	Present	Regionally restricted	3G	Expression was detected in the oligodendrocyte pregenitor cells.

Structure	Level	Pattern	Image	Note
TS24: spinal cord	Present	Regionally restricted	3H	The number of oligodendrocyte pregenitor cells is increased in the mutant and there is no apparent difference compared to wild type.

Structure	Level	Pattern	Image	Note
TS26: spinal cord	Present	Regionally restricted	3I	Expression was detected in the oligodendrocyte pregenitor cells.

Structure	Level	Pattern	Image	Note
TS26: spinal cord	Present	Regionally restricted	3J	The number of oligodendrocyte pregenitor cells is increased in the mutant and there is no apparent difference compared to wild type.

Structure	Level	Pattern	Image	Note
TS20: medial ganglionic eminence	Present	Regionally restricted	7E , 7C	Expression was high in the ventricular zone of the medial ganglionic eminence. There were positively stained migratory cells in the mantle zone of the medial ganglionic eminence.
TS20: lateral ganglionic eminence	Present		7E , 7C	Expression was detected in the lateral ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS20: medial ganglionic eminence	Present	Regionally restricted	7F , 7D	As in wild type, expression was high in the ventricular zone of the medial ganglionic eminence. There were positively stained migratory cells in the mantle zone of the medial ganglionic eminence.
TS20: lateral ganglionic eminence	Present		7D , 7F	Expression was detected in the lateral ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS22: corpus striatum	Present	Regionally restricted	8C	Expression was detected in the oligodendrocyte progenitor cells.
TS22: neocortex	Present	Regionally restricted	8C	Expression was detected in the oligodendrocyte progenitor cells.
TS22: telencephalon septum	Present	Regionally restricted	8C	Expression was detected in the oligodendrocyte progenitor cells.

Structure	Level	Pattern	Image	Note
TS22: corpus striatum	Present	Regionally restricted	8D	As in wild type, expression was detected in the oligodendrocyte progenitor cells in the mutant.
TS22: neocortex	Present	Regionally restricted	8D	As in wild type, expression was detected in the oligodendrocyte progenitor cells in the mutant.
TS22: telencephalon septum	Present	Regionally restricted	8D	As in wild type, expression was detected in the oligodendrocyte progenitor cells in the mutant.