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Gene Expression Data
Assay Details
Assay
Reference: J:125029 Pelosi M, et al., ROCK2 and its alternatively spliced isoform ROCK2m positively control the maturation of the myogenic program. Mol Cell Biol. 2007 Sep;27(17):6163-76
Assay type: RT-PCR
MGI Accession ID: MGI:5694341
Gene symbol: Rock2
Gene name: Rho-associated coiled-coil containing protein kinase 2
Probe: Rock2m-pA, Rock2m-pB
Probe preparation: labelled with FAM
Assay notes: Quantitative RT-PCR was used to obtain these results. Data were normalized for the expression of ubiquitin b. The primer set used in this assay is designed to reveal inclusion of an extra exon between exons 27 and exon 28 (referred to as 27'). Quantification of the relative abundance of the isoform is provided in Fig. 2D.
Results Image: 2C
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
heart P adult TS28: heart Weak (a) 1 µg; total RNA C57BL/6J Not Specified
brain P adult TS28: brain Absent 1 µg; total RNA C57BL/6J Not Specified
liver P adult TS28: liver Absent 1 µg; total RNA C57BL/6J Not Specified
skin P adult TS28: skin Weak (b) 1 µg; total RNA C57BL/6J Not Specified
intestine P adult TS28: intestine Absent 1 µg; total RNA C57BL/6J Not Specified
stomach P adult TS28: stomach Absent 1 µg; total RNA C57BL/6J Not Specified
testis P adult TS28: testis Absent 1 µg; total RNA C57BL/6J Male
lung P adult TS28: lung Absent 1 µg; total RNA C57BL/6J Not Specified
tibialis a. P adult TS28: tibialis anterior Strong (c) 1 µg; total RNA C57BL/6J Not Specified
gastrocn. P adult TS28: gastrocnemius muscle Strong (d) 1 µg; total RNA C57BL/6J Not Specified
quadriceps P adult TS28: quadriceps femoris muscle Strong (e) 1 µg; total RNA C57BL/6J Not Specified
soleus P adult TS28: soleus Strong (f) 1 µg; total RNA C57BL/6J Not Specified
pectoralis P adult TS28: pectoralis Strong (g) 1 µg; total RNA C57BL/6J Not Specified
latissimus d. P adult TS28: latissimus dorsi Weak (h) 1 µg; total RNA C57BL/6J Not Specified
diaphragm P adult TS28: diaphragm Strong (i) 1 µg; total RNA C57BL/6J Not Specified
Notes:
(a) This isoform is 3% of the total Rock2 transcript in this tissue.
(b) This isoform is 9% of the total Rock2 transcript in this tissue.
(c) This isoform is 31% of the total Rock2 transcript in this tissue.
(d) This isoform is 47% of the total Rock2 transcript in this tissue.
(e) This isoform is 33% of the total Rock2 transcript in this tissue.
(f) This isoform is 24% of the total Rock2 transcript in this tissue.
(g) This isoform is 46% of the total Rock2 transcript in this tissue.
(h) This isoform is 16% of the total Rock2 transcript in this tissue.
(i) This isoform is 40% of the total Rock2 transcript in this tissue.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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