Antibody:	Anti-Neogenin (AF1079)
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Level	Pattern	Image	Note
TS24: olfactory epithelium	Weak	Regionally restricted	1F	Weak staining was noted on olfactory sensory neuron axons.
TS24: main olfactory bulb external plexiform layer	Present	Regionally restricted	1F	Expression was detected in mitral cell axons in the internal and external plexiform layers.
TS24: main olfactory bulb internal plexiform layer	Present	Regionally restricted	1F	Expression was detected in mitral cell axons in the internal and external plexiform layers.
TS24: main olfactory bulb prospective glomerular layer	Weak		1F
TS24: ethmoid bone cribriform plate	Present		1F

Structure	Level	Pattern	Image	Note
TS24: olfactory tract	Present	Regionally restricted	1J	Expression was detected in axons of the lateral olfactory tract.
TS24: diencephalon	Strong	Regionally restricted	1J	Expression was detected in axons of the anterior commissure.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Strong		3F' , 3F
TS24: cerebral cortex intermediate zone	Present	Regionally restricted	3F , 3F'	Expression was on axons.
TS24: cerebral cortex ventricular layer	Present	Regionally restricted	3F , 3F'	Expression was detected in cells that had the appearance of radial glia.
TS24: cortical plate	Present	Regionally restricted	3F , 3F'	Expression was detected in cells that had the appearance of radial glia.
TS24: corpus callosum	Strong		3F

Structure	Level	Pattern	Image	Note
TS24: corpus striatum	Present	Regionally restricted	3I	Expression was on axons.
TS24: cerebral internal capsule	Present	Regionally restricted	3I	Positive axons occupied the outer part of the internal capsule.

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Strong		4F
TS28: cortical layer II	Strong		4F
TS28: cortical layer III	Strong		4F

Structure	Level	Pattern	Image	Note
TS28: cerebral internal capsule	Present
TS28: corpus callosum	Present

Structure	Level	Pattern	Image	Note
TS28: corticospinal tract	Strong		4I

Structure	Level	Pattern	Image	Note
TS24: dentate gyrus primordium	Present	Regionally restricted	6F	Expression was detected in the outer molecular layer of the dentate gyrus.
TS24: hippocampus	Present	Regionally restricted	6F	Expression was detected in the CA region and the fimbria.
TS24: hippocampus ventricular layer	Present		6F

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present	Widespread	6O
TS28: dentate gyrus granule cell layer	Present		6O
TS28: dentate gyrus molecular layer	Present		6O
TS28: dentate gyrus polymorphic layer	Present		6O
TS28: fimbria hippocampus	Present		6O
TS28: hippocampal commissure	Present		6O
TS28: hippocampus stratum lacunosum	Present		6O
TS28: hippocampus stratum oriens	Present		6O
TS28: hippocampus stratum radiatum	Present		6O

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Absent
TS28: dentate gyrus polymorphic layer	Present
TS28: hippocampus stratum radiatum	Present
TS28: fimbria hippocampus	Present			Expression was reduced as compared to P5.

Structure	Level	Pattern	Image	Note
TS24: lateral habenular nucleus	Present		8I
TS24: medial habenular nucleus	Present		8I
TS24: stria medullaris	Present		8I

Structure	Level	Pattern	Image	Note
TS24: fasciculus retroflexus	Weak		8L

Structure	Level	Pattern	Image	Note
TS28: fasciculus retroflexus	Weak		9F

Structure	Level	Pattern	Image	Note
TS24: deep cerebellar nucleus	Present		11F
TS24: cerebellum external granule cell layer	Present		11F	Expression was detected throughout the EGL and in a patch of granule cell precursors in the EGL where the presumptive rhombic lip is located.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		11L
TS28: cerebellum external granule cell layer	Present		11L
TS28: cerebellum internal granule cell layer	Present		11L

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		11R
TS28: cerebellum granule cell layer	Present		11R
TS28: cerebellum molecular layer	Present		11R	Staining may represent the dendritic processes of Purkinje cells.