Antibody:	anti-Magmas
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	The adult tissue was fixed in 10% buffered formalin.

Structure	Level	Pattern	Image	Note
TS9: trophectoderm	Present	Regionally restricted		Expression is in the trophoblast.
TS28: decidua	Present
TS9: embryo ectoderm	Present
TS9: embryo endoderm	Present

Structure	Level	Pattern	Image	Note
TS16: trophectoderm	Present
TS16: yolk sac	Present
TS28: decidua	Present
TS16: embryo ectoderm	Present
TS16: cardiac muscle tissue	Weak
TS16: future brain	Weak
TS16: future spinal cord	Weak
TS16: spinal ganglion	Weak
TS16: gut	Weak			Weak expression in intestine.
TS16: liver	Weak

Structure	Level	Pattern	Image	Note
TS20: trophectoderm	Present
TS20: yolk sac	Present
TS28: decidua	Present
TS20: skin	Present			Expression in embryonic ectoderm.
TS20: somite	Present
TS20: cardiac muscle tissue	Present
TS20: skeletal muscle	Present
TS20: brain	Present
TS20: spinal cord	Present
TS20: spinal ganglion	Present
TS20: intestine	Present
TS20: lung epithelium	Present			Expression present in bronchioles.
TS20: liver	Present

Structure	Level	Pattern	Image	Note
TS22: skin	Present			Expression in embryonic ectoderm.
TS22: cardiac muscle tissue	Weak
TS22: skeletal muscle	Present
TS22: spinal cord	Weak
TS22: spinal ganglion	Present
TS22: brain	Weak
TS22: choroid plexus	Weak
TS22: intestine	Present
TS22: bronchiole	Present
TS22: liver	Present
TS22: metanephros	Absent
TS22: nasal cavity mucosa	Absent

Structure	Level	Pattern	Image	Note
TS23: skin	Weak			Weak expression in embryonic ectoderm.
TS23: skeletal muscle	Present
TS23: brain	Weak
TS23: choroid plexus	Weak
TS23: intestine	Weak
TS23: bronchiole	Weak
TS23: liver	Present
TS23: nasal cavity mucosa	Weak
TS23: metanephros	Weak

Structure	Level	Pattern	Image	Note
TS26: skin	Weak			Weak expression in embryonic ectoderm.
TS26: cardiac muscle tissue	Absent
TS26: skeletal muscle	Absent
TS26: spinal ganglion	Weak
TS26: brain	Weak
TS26: choroid plexus	Weak
TS26: intestine	Weak
TS26: bronchiole	Absent
TS26: liver	Absent
TS26: salivary gland	Present
TS26: nasal cavity mucosa	Present
TS26: metanephros	Present
TS26: adrenal medulla	Present

Structure	Level	Pattern	Image	Note
TS28: decidua	Moderate		2A
TS9: embryo	Present	Widespread	2A
TS9: ectoplacental cone	Moderate		2A
TS9: embryo ectoderm	Moderate		2A
TS9: primitive endoderm	Moderate		2A	Expression in proximal endoderm.
TS9: parietal endoderm	Moderate		2A	Expression in distal endoderm.
TS9: Reichert's membrane	Moderate		2A

Structure	Level	Pattern	Image	Note
TS16: yolk sac endoderm	Strong		2B	Expression is present in the yolk sac epithelium.
TS16: parietal endoderm	Present		2B
TS28: decidua	Present		2B

Structure	Level	Pattern	Image	Note
TS20: atrium cardiac muscle	Strong		2C
TS20: atrioventricular canal	Strong		2C	Expression in the muscle cells.
TS20: endocardial cushion	Absent		2C	No significant expression in the stromal cells.

Structure	Level	Pattern	Image	Note
TS20: notochord	Strong		2D
TS20: somite	Strong		2D
TS20: spinal ganglion	Strong		2D

Structure	Level	Pattern	Image	Note
TS22: 4th ventricle choroid plexus	Strong	Regionally restricted	2E	Expression in the epithelium. No expression in the underlying stroma.

Structure	Level	Pattern	Image	Note
TS22: trigeminal ganglion	Strong		2F	Expression in the neuronal cells. No expression in the connective tissues.

Structure	Level	Pattern	Image	Note
TS22: skeletal muscle	Strong		2G
TS22: rib cartilage condensation	Present		2G
TS22: spinal ganglion	Present		2G

Structure	Level	Pattern	Image	Note
TS22: intestine epithelium	Strong		2H
TS22: intestine smooth muscle	Present		2H	Expression is weaker in the muscle wall.
TS22: diaphragm	Absent		2H
TS22: liver	Strong		2H

Structure	Level	Pattern	Image	Note
TS26: salivary gland epithelium	Very strong			No expression in surrounding connective tissue.
TS26: nasal cavity mucosa	Strong			Expression in the nasal mucosal epithelium in the underlying submucosal glands.

Structure	Level	Pattern	Image	Note
TS28: cardiac muscle tissue	Strong	Regionally restricted	3A	Expression in a subpopulation of fibers.

Structure	Level	Pattern	Image	Note
TS28: skeletal muscle	Strong	Widespread	3B	Expression in all fibers.

Structure	Level	Pattern	Image	Note
TS28: cardiac muscle tissue	Present	Regionally restricted	3C	Expression is localized to restricted areas within the muscle fiber.

Structure	Level	Pattern	Image	Note
TS28: renal proximal tubule	Strong		3D
TS28: medullary tubule	Absent		3D

Structure	Level	Pattern	Image	Note
TS28: renal proximal tubule	Present		3E
TS28: glomerular tuft	Absent		3E
TS28: perirenal fat	Strong		3E

Structure	Level	Pattern	Image	Note
TS28: pancreatic acinus	Present	Diffuse	3F	Expression present in acinar cells.
TS28: small intestine epithelium	Present		3F	Expression in mucosal epithelial lining.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: Leydig cell	Leydig cell	Present		4A
TS28: spermatid		Present		4A
TS28: spermatocyte		Present		4A

Structure	Level	Pattern	Image	Note
TS28: epithelium of epididymis	Strong		4B

Structure	Level	Pattern	Image	Note
TS28: ovary follicle	Absent		4C
TS28: mesenchymal stroma of ovary	Present		4C	Expression present in interfollicular stromal cells.
TS28: developing vasculature of ovary	Present		4C

Structure	Level	Pattern	Image	Note
TS28: endometrium epithelium	Weak		4D	Expression in uterine epithelium.
TS28: myometrium	Present	Single cells	4D	Expression present in occasional cells.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		4E
TS28: brain meninges	Strong		4E

Structure	Level	Pattern	Image	Note
TS28: liver	Present	Diffuse		Expression is present in most hepatocytes.
TS28: bile duct	Absent			No expression in the epithelium.
TS28: cerebral cortex	Present	Regionally restricted		Expression in neuronal but not glial cells.
TS28: lung	Trace
TS28: spleen	Trace
TS28: thymus	Trace
TS28: spinal cord	Ambiguous			No significant staining seen in the spinal cord.