Antibody:	anti-bFGF
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	fixation with Zamboni's solution.

Structure	Level	Pattern	Image	Note
TS13: yolk sac endoderm	Present	Regionally restricted	2A	Expression on the abembryonic side of endodermal cells of visceral yolk sac.
TS13: parietal endoderm	Absent		2A	No expression in parietal yolk sac.
TS13: trophoblast giant cell	Present		2A inset , 2A	Expression in giant cells of the decidua capsularis
TS13: embryo	Absent		2A

Structure	Level	Pattern	Image	Note
TS13: yolk sac endoderm	Present	Spotted	2B	Expression on abembryonic side of endodermal cells of visceral yolk sac, appearing as small or round dots.

Structure	Level	Pattern	Image	Note
TS17: yolk sac visceral endoderm	Present	Regionally restricted	2C	Expression on lateral sides of endodermal cells

Structure	Level	Pattern	Image	Note
TS19: yolk sac visceral endoderm	Present	Regionally restricted	2D	Expression on apical and lateral sides of cells. Expression also in basal region of endodermal cells .

Structure	Level	Pattern	Image	Note
TS19: yolk sac visceral endoderm	Present	Regionally restricted	3E	Expression in apical, lateral and basal regions, and intercellular spaces in the endodermal layer, endothelial cells, and mesothelium.
TS19: yolk sac mesenchyme	Present		3E	Expression in underlying mesenchymal cells, including endothelial cells, and mesothelium.