Gene Expression Data
Assay Details
Assay
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Reference:
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J:66436
Moorman AF, et al., Sensitive nonradioactive detection of mRNA in tissue sections. Novel application of the whole-mount in situ hybridization protocol. J Histochem Cytochem. 2001 Jan;49(1):1-8
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5688605
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Gene symbol:
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Myh7
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Gene name:
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myosin, heavy polypeptide 7, cardiac muscle, beta
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Probe:
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B-MHC probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3A':
embryonic day 15.0
(more )
Note: Section 5 microns thick.
Specimen
3A':
(close )
Genetic Background:
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FVB
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Age:
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embryonic day 15.0
|
Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Section 5 microns thick.
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: heart |
Trace |
|
3A'
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The signal gets stronger as the tissue gets thicker.
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Specimen
3B':
embryonic day 15.0
(more )
Note: Section 10 microns thick.
Specimen
3B':
(close )
Genetic Background:
|
FVB
|
Age:
|
embryonic day 15.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Section 10 microns thick.
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: heart |
Present |
|
3B'
|
The signal gets stronger as the tissue gets thicker.
|
Specimen
3C':
embryonic day 15.0
(more )
Note: Section 15 microns thick.
Specimen
3C':
(close )
Genetic Background:
|
FVB
|
Age:
|
embryonic day 15.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Section 15 microns thick.
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: heart |
Present |
|
3C'
|
The signal gets stronger as the tissue gets thicker.
|
|