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Gene Expression Data
Assay Details
Assay
Reference: J:66436 Moorman AF, et al., Sensitive nonradioactive detection of mRNA in tissue sections. Novel application of the whole-mount in situ hybridization protocol. J Histochem Cytochem. 2001 Jan;49(1):1-8
Assay type: RNA in situ
MGI Accession ID: MGI:5688596
Gene symbol: Irx4
Gene name: Iroquois homeobox 4
Probe: Irx4 cDNA1
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 2A: embryonic day 13.5 (more )
Note: Section hybridized at 58 degrees.
Structure Level Pattern Image Note
TS21: heart Trace 2A
Specimen 2A': embryonic day 13.5 (more )
Note: Section hybridized at 70 degrees.
Structure Level Pattern Image Note
TS21: heart Present 2A' The signal is more specific at the higher temperature.
Specimen 3A: embryonic day 15.0 (more )
Note: Section 5 microns thick.
Structure Level Pattern Image Note
TS23: heart Trace 3A The signal gets stronger as the tissue gets thicker.
Specimen 3B: embryonic day 15.0 (more )
Note: Section 10 microns thick.
Structure Level Pattern Image Note
TS23: heart Present 3B The signal gets stronger as the tissue gets thicker.
Specimen 3C: embryonic day 15.0 (more )
Note: Section 15 microns thick.
Structure Level Pattern Image Note
TS23: heart Present 3C The signal gets stronger as the tissue gets thicker.
Specimen 5A: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: heart left ventricle Present 5A
TS21: heart right ventricle Present 5A
Specimen 5E: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: left ventricle cardiac muscle Present 5E
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory