Gene Expression Data
Assay Details
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Assay
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Reference:
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J:153562
Acampora D, et al., Selective inactivation of Otx2 mRNA isoforms reveals isoform-specific requirement for visceral endoderm anteriorization and head morphogenesis and highlights cell diversity in the visceral endoderm. Mech Dev. 2009 Oct;126(10):882-97
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5662539
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Gene symbol:
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Otx2
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Gene name:
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orthodenticle homeobox 2
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Antibody:
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anti-OTX2
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Assay notes:
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Two antibodies were described MGI:1309322 and MGI:5662473; the one used in this assay was not specified.
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Results
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Specimen
S3G:
embryonic day 6.5
(more  )
Specimen
S3G:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
S3H:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Otx2tm8Asim/Otx2tm8Asim
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo |
Present |
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S3H
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There was no change in the distribution of isoforms C+D in the mutant.
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Specimen
S3I:
embryonic day 7.5
(more )
Specimen
S3I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
S3J:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Otx2tm8Asim/Otx2tm8Asim
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo |
Present |
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S3J
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There was no change in the distribution of isoforms C+D in the mutant.
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Specimen
S3K:
embryonic day 10.5
(more )
Specimen
S3K:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
S3L:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Otx2tm8Asim/Otx2tm8Asim
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: brain |
Present |
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S3L
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There was no change in the distribution of isoforms C+D in the mutant.
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Specimen
3A:
embryonic day 6.5
(more )
Specimen
3A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
3B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Otx2tm9Asim/Otx2tm9Asim
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
3C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Otx2tm9Asim/Otx2tm11Asim
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
3E:
embryonic day 7.5
(more )
Specimen
3E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: neural ectoderm |
Present |
Regionally restricted |
3E
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Expression was detected in anterior neuroectoderm.
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| TS11: mesendoderm |
Present |
Regionally restricted |
3E
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Expression was detected in axial mesendoderm.
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Specimen
3F:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Otx2tm9Asim/Otx2tm9Asim
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: neural ectoderm |
Present |
Regionally restricted |
3F
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Some cells in the anterior neural ectoderm showed a reduction of expression in the mutant.
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| TS11: mesendoderm |
Present |
Regionally restricted |
3F
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Some cells in the axial mesendoderm showed a reduction of expression in the mutant.
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Specimen
3G:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Otx2tm9Asim/Otx2tm11Asim
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: neural ectoderm |
Weak |
Regionally restricted |
3G
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Some cells in the anterior neural ectoderm showed a reduction of expression in the mutant.
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| TS11: mesendoderm |
Weak |
Regionally restricted |
3G
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Some cells in the axial mesendoderm showed a reduction of expression in the mutant.
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Specimen
5A:
embryonic day 9.5
(more )
Specimen
5A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
5F:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Otx2tm10Asim/Otx2tm11Asim
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
5K:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Otx2tm10Asim/Otx2tm11Asim
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
5P:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Otx2tm11Asim/Otx2tm11Asim
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
6A:
embryonic day 6.5
(more )
Specimen
6A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
6B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Otx2tm10Asim/Otx2tm10Asim
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
6C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Otx2tm10Asim/Otx2tm11Asim
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
7K,L:
embryonic day 7.5
(more )
Specimen
7K,L:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: mesendoderm |
Present |
Regionally restricted |
7K
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S5A
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7L
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Expression was detected in the axial mesendoderm.
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| TS11: neural ectoderm |
Present |
Regionally restricted |
S5A
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7L
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7K
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Expression was detected in anterior neural ectoderm.
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Specimen
7P,Q:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Otx2tm10Asim/Otx2tm11Asim
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
S5E:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Otx2tm10Asim/Otx2tm11Asim
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
9A:
embryonic day 6.5; Otx2tm1Pas/Otx2 +
(more )
Specimen
9A:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx2tm1Pas/Otx2+
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
9D:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx2tm1Pas/Otx2tm9Asim
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
9G:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx2tm1Pas/Otx2tm10Asim
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
9J:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx2tm1Pas/Otx2tm10Asim
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
S7A:
embryonic day 7.5
(more )
Specimen
S7A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: neural ectoderm |
Present |
Regionally restricted |
S7A
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Expression was detected in anterior neural ectoderm.
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| TS11: mesendoderm |
Present |
Regionally restricted |
S7A
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Expression was detected in axial mesendoderm.
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Specimen
S7D:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Otx2tm10Asim/Otx2tm11Asim
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Analysis Tools