Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:98439
Valasek P, et al., A dual fate of the hindlimb muscle mass: cloacal/perineal musculature develops from leg muscle cells. Development. 2005 Feb;132(3):447-58
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:5662204
|
Gene symbol:
|
Myod1
|
Gene name:
|
myogenic differentiation 1
|
Probe:
|
MyoD probe19
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
1F:
embryonic day 15.5
(more )
Specimen
1F:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: skeletal muscle |
Present |
Regionally restricted |
1F
|
Expression was detected in perineal muscles, which comprises external anal sphincter, external urethrovaginal sphincter, levator ani, bulbospongiosus and ischiocavernosus muscles.
|
Specimen
5A:
embryonic day 11.5
(more )
Specimen
5A:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: skeletal musculature |
Present |
Regionally restricted |
5A
|
Expression was detected in the ventral muscle mass of hindlimb.
|
Specimen
5B:
embryonic day 12.5
(more )
Specimen
5B:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: skeletal muscle |
Present |
Regionally restricted |
5B
|
Expression was detected in the ventrocaudal hindlimb muscle mass, which began to extend towards the genital tubercle.
|
Specimen
5C:
embryonic day 13.0
(more )
Specimen
5C:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 13.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: skeletal muscle |
Present |
Regionally restricted |
5C
|
Expression was detected in the extension of the hindlimb muscle mass that had reached the base of the tubercle.
|
Specimen
5D:
embryonic day 13.5
(more )
Specimen
5D:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: skeletal muscle |
Present |
Regionally restricted |
5D
|
Expression was detected in the muscle mass at the base of the genital tubercle that had begun to split into future single muscles.
|
Specimen
5E:
embryonic day 14.5
(more )
Specimen
5E:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: skeletal muscle |
Present |
Regionally restricted |
5E
|
Expression was detected in the perineal anlage forming separate muscle branches around the openings of the already septated cloaca.
|
Specimen
5J:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd
|
Mutant Allele(s):
|
Meox2tm1Vpa/Meox2tm1Vpa
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: skeletal muscle |
Present |
Regionally restricted |
5J
|
Expression was detected in the full complement of perineal muscles as in wild type.
|
|