Gene Expression Data
Assay Details
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Assay
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Reference:
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J:98439
Valasek P, et al., A dual fate of the hindlimb muscle mass: cloacal/perineal musculature develops from leg muscle cells. Development. 2005 Feb;132(3):447-58
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5662204
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Gene symbol:
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Myod1
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Gene name:
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myogenic differentiation 1
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Probe:
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MyoD probe19
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1F:
embryonic day 15.5
(more  )
Specimen
1F:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: skeletal muscle |
Present |
Regionally restricted |
1F
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Expression was detected in perineal muscles, which comprises external anal sphincter, external urethrovaginal sphincter, levator ani, bulbospongiosus and ischiocavernosus muscles.
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Specimen
5A:
embryonic day 11.5
(more )
Specimen
5A:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: skeletal musculature |
Present |
Regionally restricted |
5A
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Expression was detected in the ventral muscle mass of hindlimb.
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Specimen
5B:
embryonic day 12.5
(more )
Specimen
5B:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: skeletal muscle |
Present |
Regionally restricted |
5B
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Expression was detected in the ventrocaudal hindlimb muscle mass, which began to extend towards the genital tubercle.
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Specimen
5C:
embryonic day 13.0
(more )
Specimen
5C:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: skeletal muscle |
Present |
Regionally restricted |
5C
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Expression was detected in the extension of the hindlimb muscle mass that had reached the base of the tubercle.
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Specimen
5D:
embryonic day 13.5
(more )
Specimen
5D:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: skeletal muscle |
Present |
Regionally restricted |
5D
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Expression was detected in the muscle mass at the base of the genital tubercle that had begun to split into future single muscles.
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Specimen
5E:
embryonic day 14.5
(more )
Specimen
5E:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: skeletal muscle |
Present |
Regionally restricted |
5E
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Expression was detected in the perineal anlage forming separate muscle branches around the openings of the already septated cloaca.
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Specimen
5J:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Meox2tm1Vpa/Meox2tm1Vpa
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: skeletal muscle |
Present |
Regionally restricted |
5J
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Expression was detected in the full complement of perineal muscles as in wild type.
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Analysis Tools