Gene Expression Data
Assay Details
Assay
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Reference:
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J:83738
Pauley S, et al., Expression and function of FGF10 in mammalian inner ear development. Dev Dyn. 2003 Jun;227(2):203-15
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5661339
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Gene symbol:
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Fgf10
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Gene name:
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fibroblast growth factor 10
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Probe:
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Fgf10 cDNA10
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1A:
embryonic day 9.5
(more )
Specimen
1A:
(close )
Genetic Background:
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Not Specified
|
Age:
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embryonic day 9.5
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS15: otocyst |
Present |
Regionally restricted |
1A
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Expression in the entire ventral otocyst, concentrated in the lower half of the otocyst
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TS15: inner ear |
Present |
Regionally restricted |
1A
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Expression did not extend to the presumptive endolymphatic duct.
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Specimen
1B:
embryonic day 11.5
(more )
Specimen
1B:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: inner ear vestibular component |
Present |
Regionally restricted |
1B
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Strong expression in 3 distinct patches identified as future epithelia for the canal crista. Strong expression in anterior part of cochlea.
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TS19: acoustic ganglion |
Present |
Regionally restricted |
1B
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Expression in delaminating sensory neurons of cochlear and vestibular ganglia.
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Specimen
1C:
embryonic day 11.5
(more )
Specimen
1C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: inner ear vestibular component |
Present |
Regionally restricted |
1C
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Strong expression in three distince patches identified as future epithelia for the three canal crista. Less prominent expression in the cochlea.
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Specimen
1D:
embryonic day 10.5
(more )
Specimen
1D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: inner ear |
Present |
Regionally restricted |
1D
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Strong expression on anterior pole of otocyst Anterior patch of expression includes forming sensory neurons. Less extensive area of expression at the posterior pole of the otocyst.
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TS17: acoustic ganglion |
Present |
Regionally restricted |
1D
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Expression in delaminating cells of the forming cochleovestibular ganglion.
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Specimen
1E:
embryonic day 11.5
(more )
Specimen
1E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: inner ear vestibular component |
Present |
Regionally restricted |
1E
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Strong expression in three distince patches identified as future epithelia for the three canal crista. Less prominent expression in the utricle.
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Specimen
1F:
embryonic day 11.5
(more )
Specimen
1F:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: inner ear vestibular component |
Present |
Regionally restricted |
1F
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Strong expression in all three crista anlagen. Expression is throughout the columnar epithelium of the otocyst, but most concentrated in the ventricular layer.
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Specimen
2A:
postnatal day 0
(more )
Specimen
2A:
(close )
Genetic Background:
|
Not Specified
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Age:
|
postnatal day 0
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Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: crista ampullaris |
Present |
Regionally restricted |
2A
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Asymmetric distribution of expression in sensory epithelium of posterior canal cristae. Crista organs retained strong expression, with only a limited indication of a gradient falling off toward the abneural side.
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Specimen
2B:
postnatal day 0
(more )
Specimen
2B:
(close )
Genetic Background:
|
Not Specified
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Age:
|
postnatal day 0
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Sex:
|
Not Specified
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Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: utricle epithelium |
Present |
Regionally restricted |
2B
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Asymmetric distribution of expression in sensory epithelium of utricle. Higher concentration near theneural side, falling off toward the abneural side.
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Specimen
2C:
postnatal day 0
(more )
Specimen
2C:
(close )
Genetic Background:
|
Not Specified
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Age:
|
postnatal day 0
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Sex:
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Not Specified
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Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: cochlea |
Present |
Regionally restricted |
2C
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Asymmetric expression increases through greater epithelial ridge to become strongest near row of inner hair cells. Strongest expression in greater epithelial ridge. No detectable expression in Claudius cells or cochlear lateral wall.
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TS27: organ of Corti |
Present |
Regionally restricted |
2C
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Strong expression in the spiral sensory neuron. Only faintly expressed around outer hair cells.
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Specimen
2D:
postnatal day 0
(more )
Specimen
2D:
(close )
Genetic Background:
|
Not Specified
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Age:
|
postnatal day 0
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Sex:
|
Not Specified
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Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: cochlea |
Present |
Regionally restricted |
2D
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Within the greater epithelial ridge, continusous increase in expression, highest at region adjacent to inner hair cells.
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