Probe:	Sint1 cDNA probe
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present		5A
TS12: somite	Present		5A
TS12: neural ectoderm	Weak		5A

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present		5B
TS12: somite	Present		5B
TS12: trunk unsegmented mesenchyme	Absent		5B
TS12: allantois	Weak		5B

Structure	Level	Pattern	Image	Note
TS14: mesenchyme derived from lateral plate	Present	Regionally restricted		Lateral plate expression focused in developing forelimb and hindlimb buds.
TS14: 1st branchial arch	Present
TS14: 2nd branchial arch	Present
TS14: otic pit	Present
TS14: somite	Weak
TS14: mesenchyme derived from neural crest	Strong	Regionally restricted		Strong segmental expression in neural crest cells giving rise to dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS15: tail somite	Present	Regionally restricted	5C	In tailbud, expression in newly formed somites.

Structure	Level	Pattern	Image	Note
TS15: branchial arch mesenchyme	Strong		5D
TS15: limb mesenchyme	Strong		5D
TS15: olfactory placode	Strong		5D

Structure	Level	Pattern	Image	Note
TS15: branchial arch mesenchyme	Strong		5E
TS15: dorsal root ganglion	Strong		5E
TS15: limb mesenchyme	Strong		5E

Structure	Level	Pattern	Image	Note
TS17: limb	Strong	Regionally restricted	5F	Strong expression more confined to limbs and newoly formed dorsal root ganglia of tail.
TS17: tail mesenchyme derived from neural crest	Strong	Regionally restricted	5F	Strong expression more confined to limbs and newoly formed dorsal root ganglia of tail.

Structure	Level	Pattern	Image	Note
TS19: autopod	Present	Regionally restricted	5G	Expression concentrating around developing digits.

Structure	Level	Pattern	Image	Note
TS20: autopod	Present	Regionally restricted	5H	Expression concentrating around developing digits.
TS20: mammary bud	Present		5H
TS20: vibrissa follicle	Present		5H

Structure	Level	Pattern	Image	Note
TS22: autopod	Strong	Regionally restricted	5I	Expression concentrating around developing digits.

Structure	Level	Pattern	Image	Note
TS15: branchial arch mesenchyme	Strong		5J
TS15: neural crest	Strong		5J

Structure	Level	Pattern	Image	Note
TS15: dorsal root ganglion	Present		5K
TS15: sclerotome	Present		5K

Structure	Level	Pattern	Image	Note
TS15: dorsal root ganglion	Present		5L
TS15: sclerotome	Present		5L
TS15: dermomyotome	Present	Regionally restricted	5L	No expression in outer cells of dermomyotome.
TS15: nephric duct, mesonephric portion	Present	Regionally restricted	5L	Weak to moderate expression in mesonephric duct.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present		5M

Structure	Level	Pattern	Image	Note
TS15: embryo	Present		5N

Structure	Level	Pattern	Image	Note
TS15: tail unsegmented mesenchyme	Absent		5O

Structure	Level	Pattern	Image	Note
TS15: gut	Present
TS15: neural ectoderm	Present
TS15: notochord	Present
TS15: heart	Absent
TS15: surface ectoderm	Absent