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Gene Expression Data
Assay Details
Assay
Reference: J:149243 Wilhelm D, et al., Antagonism of the testis- and ovary-determining pathways during ovotestis development in mice. Mech Dev. 2009 May-Jun;126(5-6):324-36
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5646132
Gene symbol: Foxl2
Gene name: forkhead box L2
Antibody: Anti-Foxl2 (C terminus)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: Two antibodies were described in the reference for this antibody - MGI:5618299 and MGI:5640581. Either could have been used in this assay.
Results
Specimen 2I: embryonic day 13.5; X/SryPOS (more )
Note: XY ovary. Double labeled: green - Foxl2; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS21: ovary Present 2I Expression was detected along the whole length of the gonad.
Specimen 4A: embryonic day 11.5 (more )
Note: XY. Double labeled: green - Foxl2; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS19: gonad Absent 4A
Specimen 4B: embryonic day 12.5 (more )
Note: XY. Double labeled: green - Foxl2; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS20: testis Absent 4B
Specimen 4C: embryonic day 13.5 (more )
Note: XY. Double labeled: green - Foxl2; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS21: testis Absent 4C
Specimen 4D: embryonic day 11.5; X/SryPOS (more )
Note: XY ovotestes. Double labeled: green - Foxl2; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS19: gonad Present Regionally restricted 4D Expression was predominantly at the poles in ovotestes.
Specimen 4E: embryonic day 12.5; X/SryPOS (more )
Note: XY ovotestes. Double labeled: green - Foxl2; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS20: gonad Present Regionally restricted 4E Expression was predominantly at the poles in ovotestes. In 14 of 29 ovotestis, expression was not completely excluded from the center where the testicular program was active.
Specimen 4F: embryonic day 13.5; X/SryPOS (more )
Note: XY ovotestes. Double labeled: green - Foxl2; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS21: gonad Present Regionally restricted 4F Expression was predominantly at the poles in ovotestes. In 14 of 29 ovotestis, expression was not completely excluded from the center where the testicular program was active.
Specimen 4G: embryonic day 11.5 (more )
Note: XX. Double labeled: green - Foxl2; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS19: gonad Strong 4G
Specimen 4H: embryonic day 12.5 (more )
Note: XX. Double labeled: green - Foxl2; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS20: ovary Strong 4H
Specimen 4I: embryonic day 13.5 (more )
Note: XX. Double labeled: green - Foxl2; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS21: ovary Strong 4I
Specimen 5A right: embryonic day 14.5; X/SryPOS (more )
Note: XY ovotestes. Double labeled: green - Foxl2; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS22: gonad Present Regionally restricted 5A right Expression was detected in ovarian tissue at the poles in ovotestes and in morphological testicular tissue in the center.
Specimen 6E: embryonic day 11.5; X/SryPOS (more )
Note: XY ovotestes. Double labeled: green - Foxl2; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS19: gonad Present Regionally restricted 6E Expression was detected in regions in which the ovarian pathway was initiated in the ovotestis.
Specimen 6F: embryonic day 12.5; X/SryPOS (more )
Note: XY ovotestes. Double labeled: green - Foxl2; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS20: gonad Present Regionally restricted 6F Expression was detected in regions in which the ovarian pathway was initiated in the ovotestis.
Specimen 6G: embryonic day 13.5; X/SryPOS (more )
Note: XY ovotestes. Double labeled: green - Foxl2; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS21: gonad Present Regionally restricted 6G Expression was detected in regions in which the ovarian pathway was initiated in the ovotestis.
Specimen 6H: embryonic day 14.5; X/SryPOS (more )
Note: XY ovotestes. Double labeled: green - Foxl2; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS22: gonad Present Regionally restricted 6H Expression was detected in regions in which the ovarian pathway was initiated in the ovotestis.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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