Gene Expression Data
Assay Details
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Assay
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Reference:
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J:149243
Wilhelm D, et al., Antagonism of the testis- and ovary-determining pathways during ovotestis development in mice. Mech Dev. 2009 May-Jun;126(5-6):324-36
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5646130
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Gene symbol:
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Sox9
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Gene name:
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SRY (sex determining region Y)-box 9
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Antibody:
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anti-Sox9
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Assay notes:
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Two antibodies were described in the reference for this antibody - MGI:3577970 and MGI:5645945. Either could have been used in this assay.
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Results
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Specimen
2H:
embryonic day 13.5; X/ SryPOS
(more  )
Note: XY ovary. Double labeled: green - Sox9; red - Cdh1 (assay MGI:5646129).
Specimen
2H:
(close  )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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X/SryPOS
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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XY ovary. Double labeled: green - Sox9; red - Cdh1 (assay MGI:5646129).
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Specimen
3A:
embryonic day 11.5
(more )
Note: XY. Double labeled: green - Sox9; red - Cdh1 (assay MGI:5646129).
Specimen
3A:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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XY. Double labeled: green - Sox9; red - Cdh1 (assay MGI:5646129).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: urogenital ridge |
Present |
Regionally restricted |
3A
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Expression was detected in Sertoli cell precursors in the center/anterior pole of the genital ridge.
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Specimen
3B:
embryonic day 12.5
(more )
Note: XY. Double labeled: green - Sox9; red - Cdh1 (assay MGI:5646129).
Specimen
3B:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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XY. Double labeled: green - Sox9; red - Cdh1 (assay MGI:5646129).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: Sertoli cell |
Sertoli cell |
Present |
Regionally restricted |
3B
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Expression was detected in Sertoli cells found to enclose Cdh1-positive germ cells.
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Specimen
3C:
embryonic day 13.5
(more )
Note: XY. Double labeled: green - Sox9; red - Cdh1 (assay MGI:5646129).
Specimen
3C:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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XY. Double labeled: green - Sox9; red - Cdh1 (assay MGI:5646129).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: primary sex cord |
Present |
Regionally restricted |
3C
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All positive cells were incorporated into testis cords.
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Specimen
3D:
embryonic day 11.5; X/ SryPOS
(more )
Note: XY ovotestes. Double labeled: green - Sox9; red - Cdh1 (assay MGI:5646129).
Specimen
3D:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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X/SryPOS
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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XY ovotestes. Double labeled: green - Sox9; red - Cdh1 (assay MGI:5646129).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: urogenital ridge |
Present |
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3D
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The onset of expression in the genital ridges was similar to the wild type.
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Specimen
3E:
embryonic day 12.5; X/ SryPOS
(more )
Note: XY ovotestes. Double labeled: green - Sox9; red - Cdh1 (assay MGI:5646129).
Specimen
3E:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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X/SryPOS
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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XY ovotestes. Double labeled: green - Sox9; red - Cdh1 (assay MGI:5646129).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: urogenital ridge |
Present |
Regionally restricted |
3E
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Expression was either not up-regulated (n=6) or was present in the center but not at the poles (n=8) of the genital ridge.
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Specimen
3F:
embryonic day 13.5; X/ SryPOS
(more )
Note: XY ovotestes. Double labeled: green - Sox9; red - Cdh1 (assay MGI:5646129).
Specimen
3F:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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X/SryPOS
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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XY ovotestes. Double labeled: green - Sox9; red - Cdh1 (assay MGI:5646129).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: primary sex cord |
Present |
Regionally restricted |
3F
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In the central region of the ovotestis, positive cells assembled into testicular cords, enclosing only those germ cells resident in this region.
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Specimen
3H/I:
embryonic day 12.5; X/ SryPOS
(more )
Note: XY ovotestes. Double labeled: green - Sox9; red - Sry (assay MGI:5646219).
Specimen
3H/I:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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X/SryPOS
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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XY ovotestes. Double labeled: green - Sox9; red - Sry (assay MGI:5646219).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: gonad |
Present |
Regionally restricted |
3H
,
3I
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Expression was up-regulated in the center, and in some cases at the posterior (this specimen) or anterior pole.
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Specimen
5A left:
embryonic day 14.5; X/ SryPOS
(more )
Note: XY ovotestes. Double labeled: green - Sox9; red - Cdh1 (assay MGI:5646129).
Specimen
5A left:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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X/SryPOS
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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XY ovotestes. Double labeled: green - Sox9; red - Cdh1 (assay MGI:5646129).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: primary sex cord |
Present |
Regionally restricted |
5A left
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Expression was detected in the morphological testicular tissue in the center of the ovotestis.
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Specimen
5B:
embryonic day 13.5; X/ SryPOS
(more )
Note: XY ovotestes. Double labeled: green - Sox9; red - Foxl2 (assay MGI:5646132).
Specimen
5B:
(close )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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X/SryPOS
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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XY ovotestes. Double labeled: green - Sox9; red - Foxl2 (assay MGI:5646132).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: primary sex cord |
Present |
Regionally restricted |
5B
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Expression was detected in the morphological testicular tissue in the center of the ovotestis. Individual positive cells in this region did not co-express with Foxl2.
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