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Gene Expression Data
Assay Details
Assay
Reference: J:149243 Wilhelm D, et al., Antagonism of the testis- and ovary-determining pathways during ovotestis development in mice. Mech Dev. 2009 May-Jun;126(5-6):324-36
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5646128
Gene symbol: Sry
Gene name: sex determining region of Chr Y
Antibody: Anti-Sry
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2A: embryonic day 11.5 (more )
Note: XY. Double labeled: green - Sry; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS19: urogenital ridge Present 2A Expression was along the whole length of the genital ridge.
Specimen 2B: embryonic day 12.5 (more )
Note: XY. Double labeled: green - Sry; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS20: testis Present Regionally restricted 2B Expression was present in only a few cells throughout the testis, mostly at the posterior pole.
Specimen 2C: embryonic day 13.5 (more )
Note: XY. Double labeled: green - Sry; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS21: testis Absent 2C
Specimen 2D: embryonic day 11.5; X/SryPOS (more )
Note: XY. Double labeled: green - Sry; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS19: urogenital ridge Present 2D Expression was along the whole length of the genital ridge.
Specimen 2E: embryonic day 12.5; X/SryPOS (more )
Note: XY ovotestis. Double labeled: green - Sry; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS20: gonad Present 2E Ovotestes retained more expressing cells compared to the wild type testis.
Specimen 2F: embryonic day 13.5; X/SryPOS (more )
Note: XY ovotestis. Double labeled: green - Sry; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS21: gonad Present Single cells 2F A few positive cells were detectable.
Specimen 2G: embryonic day 13.5; X/SryPOS (more )
Note: XY ovary. Double labeled: green - Sry; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS21: ovary Present Single cells 2G There was a delay in the down-regulation of expression in the XY<POS> ovaries.
Specimen not shown: embryonic day 14.5; X/SryPOS (more )
Note: XY ovotestis.
Structure Level Pattern Image Note
TS22: gonad Absent
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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