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Gene Expression Data
Assay Details
Assay
Reference: J:221424 Miwa H, et al., Generation and characterization of PDGFRalpha-GFPCreER(t2) knock-In mouse line. Genesis. 2015 May;53(5):329-36
Assay type: Recombinase reporter
MGI Accession ID: MGI:5645256
Gene symbol: Pdgfra
Gene name: platelet derived growth factor receptor, alpha polypeptide
Reporter: RFP
Antibody: anti-RFP (Chromotek 5F8)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Assay notes: Induction was achieved by intraperitoneal injection of 1 mg/ml tamoxifen dissolved in corn oil at 8 weeks.
Results
Specimen 2e skin: postnatal week 9; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Pdgfratm1.1(EGFP/cre/ERT2)Hyma/Pdgfra+ (more )
Note: Conditional mutant. Double labeled: red - recombinase reporter; green Pdgfra protein.
Structure Level Pattern Image Note
TS28: dermis Present Single cells 2e skin merge , 2e skin Tomato Expression is present in fibroblasts.
Specimen 2e lung: postnatal week 9; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Pdgfratm1.1(EGFP/cre/ERT2)Hyma/Pdgfra+ (more )
Note: Conditional mutant. Double labeled: red - recombinase reporter; green Pdgfra protein.
Structure Level Pattern Image Note
TS28: lung parenchyma Present 2e lung Tomato , 2e lung merge Expression is present in the mesenchyme.
Specimen 2e muscle: postnatal week 9; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Pdgfratm1.1(EGFP/cre/ERT2)Hyma/Pdgfra+ (more )
Note: Conditional mutant. Double labeled: red - recombinase reporter; green Pdgfra protein.
Structure Level Pattern Image Note
TS28: skeletal muscle Present Regionally restricted 2e muscle Tomato , 2e muscle merge Expression is present in satellite cells.
Specimen 2e brain: postnatal week 9; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Pdgfratm1.1(EGFP/cre/ERT2)Hyma/Pdgfra+ (more )
Note: Conditional mutant. Double labeled: red - recombinase reporter; green Pdgfra protein.
Structure Level Pattern Image Note
TS28: brain Present Single cells 2e brain merge , 2e brain Tomato Expression is present in glia.
Specimen Supp skin: postnatal week 9; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Pdgfratm1.1(EGFP/cre/ERT2)Hyma/Pdgfra+ (more )
Note: Conditional mutant. This tissue was unfixed.
Structure Level Pattern Image Note
TS28: dermis Present Single cells Supplemental_skin Expression is present in fibroblasts.
Specimen Supp lung: postnatal week 9; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Pdgfratm1.1(EGFP/cre/ERT2)Hyma/Pdgfra+ (more )
Note: Conditional mutant. This tissue was unfixed.
Structure Level Pattern Image Note
TS28: lung parenchyma Present Supplemental_lung Expression is present in the mesenchyme.
Specimen Supp muscle: postnatal week 9; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Pdgfratm1.1(EGFP/cre/ERT2)Hyma/Pdgfra+ (more )
Note: Conditional mutant. This tissue was unfixed.
Structure Level Pattern Image Note
TS28: skeletal muscle Present Single cells Supplemental_muscle Expression is present in satellite cells.
Specimen Supp brain: postnatal week 9; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Pdgfratm1.1(EGFP/cre/ERT2)Hyma/Pdgfra+ (more )
Note: Conditional mutant. This tissue was unfixed.
Structure Level Pattern Image Note
TS28: brain Present Single cells Supplemental_brain Expression is present in glia.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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01/28/2026
MGI 6.24 		The Jackson Laboratory