Pdgfra In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:221424 Miwa H, et al., Generation and characterization of PDGFRalpha-GFPCreER(t2) knock-In mouse line. Genesis. 2015 May;53(5):329-36
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:5644481
Gene symbol:	Pdgfra
Gene name:	platelet derived growth factor receptor, alpha polypeptide

Reporter:	GFP
Antibody:	anti-GFP (Nacalai)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

3a GFP ©

3a merge ©

Specimen 3a: embryonic day 7.5; Pdgfra^{tm1.1(EGFP/cre/ERT2)Hyma}/Pdgfra⁺ (more

)
Note: Double labeled: green - Pdgfra reporter; red - Pdgfra (assay MGI:5645236).

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present		3a merge , 3a GFP

3b GFP lower ©

3b GFP upper ©

Specimen 3b: embryonic day 9.5; Pdgfra^{tm1.1(EGFP/cre/ERT2)Hyma}/Pdgfra⁺ (more

)
Note: Double labeled: green - Pdgfra reporter; red - Pdgfra (assay MGI:5645236).

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present	Regionally restricted	3b GFP center , 3b GFP upper , 3b merge upper , 3b merge center	Expression is present in facial mesenchyme.
TS15: branchial arch	Present		3b GFP upper , 3b merge center , 3b merge upper , 3b GFP center
TS15: somite	Present		3b GFP lower , 3b merge upper , 3b GFP upper , 3b merge lower

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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