Antibody:	Anti-Sumo2/3
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	The antibody used in the assay recognizes two family members.

Structure	Level	Pattern	Image	Note
TS24: lens epithelium	Strong		1Ad , 1Ac , 1Ad1 , 1Ad2
TS24: lens fiber	Weak	Regionally restricted	1Ad2 , 1Ad , 1Ad1 , 1Ac	There was a decreased signal in elongating primary lens fiber cells, and eventually the signal disappeard in terminally differentiated cells.

Structure	Level	Pattern	Image	Note
TS19: lens vesicle anterior epithelium	Present		4A Sumo2/3 , 4A Merge lower , 4A Sumo2/3/Sp1	Expression co-localized with Sp1.
TS19: lens vesicle	Present	Regionally restricted	4A Sumo2/3 , 4A Merge lower , 4A Sumo2/3/Sp1	Co-localization with Sp1 was greatly reduced in the posterior cells undergoing differentiation into primary lens fibers.

Structure	Level	Pattern	Image	Note
TS15: eye	Present		S1E , S1E'

Structure	Level	Pattern	Image	Note
TS19: lens vesicle	Strong	Ubiquitous	S1F , S1F'

Structure	Level	Pattern	Image	Note
TS21: lens epithelium	Present		S1G' , S1G2 , S1G , S1G1
TS21: lens fiber	Present	Regionally restricted	S1G1 , S1G' , S1G2 , S1G	Expression was in early differentiating lens fiber cells.

Structure	Level	Pattern	Image	Note
TS27: lens epithelium	Present		S1H , S1H1 , S1H'1 , S1H'
TS27: lens fiber	Present	Regionally restricted	S1H' , S1H1 , S1H , S1H'1	Expression was detected only in a small portion of lens fiber cells at the transition zone (H'1), but not in lens fiber cells undergoing terminal differentiation (H1).

Structure	Level	Pattern	Image	Note
TS28: ciliary body	Weak		S1J
TS28: lens epithelium	Weak		S1J
TS28: lens fiber	Absent		S1J