Antibody:	Anti-Sumo1
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS24: lens anterior epithelium	Present	Regionally restricted	1Ab , 1Ab2 , 1Aa	Expression was homogenous in nuclei of lens epithelial cells at the anterior portion of the lens.
TS24: lens fiber	Present	Regionally restricted	1Aa , 1Ab1 , 1Ab	Expression was detected in elongating lens fiber cells. In cells moving out of the transition zone and in deeper layers, label was primarily in the nuclear periphery.

Structure	Level	Pattern	Image	Note
TS19: lens vesicle anterior epithelium	Present	Uniform	4A Merge upper , 4A Sumo1 , 4A Sumo1/Sp1	Expression co-localized with Sp1.
TS19: lens vesicle	Present	Regionally restricted	4A Sumo1 , 4A Sumo1/Sp1 , 4A Merge upper	Expression co-localized with Sp1 in the posterior differentiating cells of the lens vesicle.

Structure	Level	Pattern	Image	Note
TS15: eye	Present	Patchy	S1A' , S1A

Structure	Level	Pattern	Image	Note
TS19: lens vesicle	Strong	Ubiquitous	S1B' , S1B

Structure	Level	Pattern	Image	Note
TS21: lens epithelium	Present		S1C , S1C1 , S1C2 , S1C'
TS21: lens fiber	Present		S1C , S1C1 , S1C2 , S1C'

Structure	Level	Pattern	Image	Note
TS27: lens epithelium	Present		S1D'1 , S1D , S1D1 , S1D'
TS27: lens fiber	Present		S1D'1 , S1D , S1D1 , S1D'

Structure	Level	Pattern	Image	Note
TS28: lens epithelium	Present		S1I' , S1I	Expression was mainly seen in lens epithelial cells.
TS28: lens fiber	Present	Regionally restricted	S1I' , S1I	There was a detectable level of expression in the differentiating lens fiber cells of the transition zone.