Gene Expression Data
Assay Details
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Assay
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Reference:
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J:46409
Hirsch MR, et al., Control of noradrenergic differentiation and Phox2a expression by MASH1 in the central and peripheral nervous system. Development. 1998 Feb;125(4):599-608
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5636467
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Gene symbol:
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Phox2a
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Gene name:
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paired-like homeobox 2a
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Results
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Specimen
1A:
embryonic day 10.5; Ascl1tm1And/Ascl1 +
(more  )
Note: Section through the cervical region. Double labeled: orange - Phox2a; black - Ret (assay MGI:5636466).
Specimen
1A:
(close  )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Section through the cervical region. Double labeled: orange - Phox2a; black - Ret (assay MGI:5636466).
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Specimen
1B:
embryonic day 10.5; Ascl1tm1And/ Ascl1tm1And
(more )
Note: Section through the cervical region. Double labeled: orange - Phox2a; black - Ret (assay MGI:5636466).
Specimen
1B:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Section through the cervical region. Double labeled: orange - Phox2a; black - Ret (assay MGI:5636466).
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Specimen
1E:
embryonic day 10.5; Ascl1tm1And/Ascl1 +
(more )
Note: Section through the thoracic region.
Specimen
1E:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Section through the thoracic region.
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Specimen
1F:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Section through the thoracic region.
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Specimen
1M:
embryonic day 13.5; Ascl1tm1And/Ascl1 +
(more )
Specimen
1M:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
1N:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: inferior cervical ganglion |
Present |
Regionally restricted |
1N
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The few surviving sympathoblasts are labelled in the rudimentary ganglion in the mutant.
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Specimen
2C:
embryonic day 13.5; Ascl1tm1And/Ascl1 +
(more )
Specimen
2C:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
2D:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
2I:
embryonic day 13.5; Ascl1tm1And/Ascl1 +
(more )
Specimen
2I:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
2J:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
3C:
embryonic day 16.5; Ascl1tm1And/Ascl1 +
(more )
Specimen
3C:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
3D:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: sphenopalatine ganglion |
Weak |
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3D
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The few remaining cells in the mutant atrophic sphenopalatine ganglion show weak expression.
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Specimen
4C:
embryonic day 13.5; Ascl1tm1And/Ascl1 +
(more )
Specimen
4C:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: esophagus |
Present |
Regionally restricted |
4C
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Expression was detected in the myenteric plexus of the stomach and lower esophagus.
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| TS21: stomach |
Present |
Regionally restricted |
4C
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Expression was detected in the myenteric plexus of the stomach and lower esophagus.
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Specimen
4D:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: stomach |
Present |
Regionally restricted |
4D
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Expression was detected in the myenteric plexus of the stomach, as in wild type.
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| TS21: esophagus |
Absent |
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4D
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The presumptive myenteric neurons of the lower esophagus were negative.
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Specimen
4G:
embryonic day 13.5; Ascl1tm1And/Ascl1 +
(more )
Specimen
4G:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: esophagus |
Present |
Regionally restricted |
4G
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Expression was detected in the myenteric plexus of the upper esophagus.
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Specimen
4H:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: esophagus |
Absent |
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4H
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There was no staining in the myenteric plexus of the upper esophagus.
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Specimen
5E:
postnatal newborn; Ascl1tm1And/Ascl1 +
(more )
Specimen
5E:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1+
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Specimen
5F:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Specimen
5G:
embryonic day 13.5; Ascl1tm1And/Ascl1 +
(more )
Specimen
5G:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: pons |
Present |
Regionally restricted |
5G
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Expression was detected in the locus coeruleus anlage.
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| TS21: brainstem |
Present |
Regionally restricted |
5G
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Expression was detected in the reticular formation.
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Specimen
5H:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: brainstem |
Present |
Regionally restricted |
5H
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Expression was detected in the reticular formation.
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| TS21: pons |
Ambiguous |
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5H
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There was no expression in the expected location of the locus coeruleus neurons, which appear to never develop in the mutant.
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Specimen
7I:
embryonic day 13.5; Ascl1tm1And/Ascl1 +
(more )
Specimen
7I:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: alar columns |
Present |
Regionally restricted |
7I
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Expression was found in a discrete column of cells in the lateral neural tube just dorsal to the sulcus limitans.
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Specimen
7J:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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