Antibody:

Anti-Lyve1 (103-PA50)

Structure	Level	Pattern	Image	Note
TS15: cardiac muscle tissue	Present	Regionally restricted	4B	Expression was detected on the endocardial side of the myocardium at the leading edge of the nascent interventricular septum.

Structure	Level	Pattern	Image	Note
TS15: cardiac muscle tissue	Present		4F , 4E	There was no co-localization with the cardiac myocyte marker MF-20.

Structure	Level	Pattern	Image	Note
TS15: cardiac muscle tissue	Present		4G	There was no co-localization with the lymphatic marker Podoplanin.

Structure	Level	Pattern	Image	Note
TS15: cardiac muscle tissue	Present		4I , 4K	Expression co-localized with a subset of cells positive for the endothelial cell marker CD31/Pecam1.

Structure	Level	Pattern	Image	Note
TS21: visceral pericardium	Present	Regionally restricted	5B	Expression was in epicardial cells on the great vessels.

Structure	Level	Pattern	Image	Note
TS21: cardiac muscle tissue	Present	Regionally restricted	5C	Expression was in myocardial vessels.

Structure	Level	Pattern	Image	Note
TS21: visceral pericardium	Present	Regionally restricted	5D	Expression was in ventricular epicardium.

Structure	Level	Pattern	Image	Note
TS21: visceral pericardium	Present	Regionally restricted	6A	Expression was in mesothelial cells of the epicardium.

Structure	Level	Pattern	Image	Note
TS21: heart mesenchyme	Present	Regionally restricted	6B	Expression was in the subepicardial mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: cardiac muscle tissue	Present		6C

Structure	Level	Pattern	Image	Note
TS23: great vessel of heart	Present		7B	Large numbers of positive cells were detected on the great vessels. Some cells were arranged in a network.
TS23: heart atrium	Present		7B
TS23: heart ventricle	Present		7B

Structure	Level	Pattern	Image	Note
TS23: lymphatic system	Present	Regionally restricted	7D	Expression was detected in the lymphatics on the great vessels. Expression co-localized with Prox1.

Structure	Level	Pattern	Image	Note
TS23: cardiac muscle tissue	Present		7F	Immunopositive cells in these tissues did not co-localize with Prox1.
TS23: heart ventricle	Present		7F	Immunopositive cells in these tissues did not co-localize with Prox1.

Structure	Level	Pattern	Image	Note
TS28: lymphatic vessel	Present	Regionally restricted	8C	Expression was in lymphatic vessels at the atrioventricular junction.

Structure	Level	Pattern	Image	Note
TS28: lymphatic vessel	Present	Regionally restricted	8G	Expression was in the epicardial lymphatic vessels covering the ventricles.
TS28: visceral pericardium	Present	Regionally restricted	8G	Expression was in the epicardial lymphatic vessels covering the ventricles.

Structure	Level	Pattern	Image	Note
TS28: ventricle cardiac muscle	Present		8K

Structure	Level	Pattern	Image	Note
TS28: lymphatic vessel	Present	Regionally restricted	S2A	Expression was detected in the epicardial lymphatics, which was co-labeled with Pecam1.

Structure	Level	Pattern	Image	Note
TS28: ventricle cardiac muscle	Present	Regionally restricted	S2B	Expression was in the vessels within the ventricular myocardium. Some cells were co-labeled with Pecam1.