Gene Expression Data
Assay Details
Assay
|
Reference:
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J:40904
Bouillet P, et al., Developmental expression pattern of Stra6, a retinoic acid-responsive gene encoding a new type of membrane protein. Mech Dev. 1997 May;63(2):173-86
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5633927
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Gene symbol:
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Stra6
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Gene name:
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stimulated by retinoic acid gene 6
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Antibody:
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Stra6 sntibody
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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|
Results
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Specimen
4A:
postnatal adult
(more )
Specimen
4A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Male
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Type:
|
section
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Fixation:
|
Acetone
|
Specimen
4C:
(close )
Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
|
Rarbtm1Ipc/Rarbtm1Ipc
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Age:
|
postnatal adult
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Acetone
|
Specimen
4E:
(close )
Genetic Background:
|
involves: 129S2/SvPas
|
Mutant Allele(s):
|
Raratm1Ipc/Raratm1Ipc
|
Age:
|
postnatal adult
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Acetone
|
Specimen
5A:
postnatal adult
(more )
Note: Fixative was 4% paraformaldehyde, 0.2% picric acid and 0.1% glutaraldehyde. Stained sections were fixed with 5% glutaraldehyde in PBS, postfixed in 1% osmium tetroxide and infiltrated with Epon resin.
Specimen
5A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal adult
|
Sex:
|
Male
|
Type:
|
section
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Fixation:
|
Other - See Notes
|
Embedding:
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Resin/plastic
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Note:
|
Fixative was 4% paraformaldehyde, 0.2% picric acid and 0.1% glutaraldehyde. Stained sections were fixed with 5% glutaraldehyde in PBS, postfixed in 1% osmium tetroxide and infiltrated with Epon resin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: Sertoli cell |
Sertoli cell |
Present |
Regionally restricted |
5A
|
Expression is in the plasma membrane of the basal pole of Sertoli cells.
|
TS28: spermatogonium |
|
Absent |
|
5A
|
|
Specimen
5B:
postnatal adult
(more )
Note: Electron microscopy. Fixative was 4% paraformaldehyde, 0.2% picric acid and 0.1% glutaraldehyde. Stained sections were fixed with 5% glutaraldehyde in PBS, postfixed in 1% osmium tetroxide and infiltrated with Epon resin.
Specimen
5B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal adult
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Resin/plastic
|
Note:
|
Electron microscopy. Fixative was 4% paraformaldehyde, 0.2% picric acid and 0.1% glutaraldehyde. Stained sections were fixed with 5% glutaraldehyde in PBS, postfixed in 1% osmium tetroxide and infiltrated with Epon resin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: Sertoli cell |
Sertoli cell |
Present |
Regionally restricted |
5B
|
Expression is in the plasma membrane of the basal pole of Sertoli cells.
|
TS28: spermatogonium |
|
Absent |
|
5B
|
|
Specimen
6A:
embryonic day 17.5
(more )
Specimen
6A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Specimen
6B:
postnatal adult
(more )
Specimen
6B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Specimen
not shown, A:
postnatal adult
(more )
Specimen
not shown, A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: optic nerve |
Present |
Regionally restricted |
|
Expression is in the meninges surrounding the optic nerve.
|
TS28: meninges |
Present |
|
|
|
TS28: brain blood vessel |
Present |
Regionally restricted |
|
Expression is in capillaries in the preoptic zone and in the medial cerebellar and ventral cochlear nuclei.
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Specimen
7E:
postnatal adult
(more )
Specimen
7E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Specimen
7F:
postnatal adult
(more )
Specimen
7F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
|