Antibody:	Anti-basic FGF
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Two antibodies were described. Authors state the antibodies resulted in identical localization patterns. Results were reported together without specifying which antibody was used. See also MGI:5633878 for details of the second antibody.

Structure	Level	Pattern	Image	Note
TS28: uterus	Present	Regionally restricted	1	Expression was detected in the inter-implantation sites. Expression was detected in basal laminae associated with the uterine lumen, glandular epithelial cells, endothelial cells of vascular elements and the mesothelial covering of the uterus.
TS28: uterus stroma	Present	Regionally restricted	1	Expression was detected in the extracellular matrix of the uterine stroma.
TS28: endometrium glandular epithelium	Absent		1
TS28: uterus lumen	Absent		1
TS28: endometrium luminal epithelium	Present	Diffuse	1
TS28: myometrium inner circular smooth muscle layer	Present		1
TS28: myometrium outer longitudinal smooth muscle layer	Present		1
TS28: adventitia of uterus	Present	Regionally restricted	1	Expression was detected in the extracellular matrix of the perimetrium.

Structure	Level	Pattern	Image	Note
TS28: endometrium luminal epithelium	Present		2
TS28: uterus	Present	Regionally restricted	3 , 2	The residual luminal basal lamina stained positive.

Structure	Level	Pattern	Image	Note
TS6: blastocyst	Absent		2 , 3	There was little or no staining within the hatched, non-invasive blastocyst.

Structure	Level	Pattern	Image	Note
TS10: embryo	Present		4 , 5
TS10: ectoplacental cone	Present	Diffuse	4 , 5

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted	5 , 4	There was no expression in the primary decidual zone. Expression was detected in the secondary decidual zone.
TS28: uterus stroma	Present		4	Expression was in the extracellular matrix.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted	6	Expression was detected in the secondary decidual zone in a pericellular fashion surrounding individual decidual cells. Basal laminae associated with blood vessels were also positive.

Structure	Level	Pattern	Image	Note
TS13: embryo	Present		7
TS13: ectoplacental cone	Absent		7

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted	7	Decidual cells in the mesometrial region contained diffuse cytoplasmic staining.
TS28: mesometrium	Present	Regionally restricted	7	Decidual cells in the mesometrial region contained diffuse cytoplasmic staining.

Structure	Level	Pattern	Image	Note
TS13: embryo	Present		8
TS13: trophoblast giant cell	Absent		8	Lateral giant trophoblast cells were negative.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted	8	Expression was detected in the regressing primary decidual zone in the cell cytoplasm and small amounts in extracellular spaces.