Antibody:	anti-EIIIB FN
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Strong	Regionally restricted	1D	Expressed in both punctate intracellular and fibrillar intercellular patterns.
TS11: exocoelomic cavity	Strong	Regionally restricted	1D	Expressed in cells lining the exocoelomic cavity.
TS11: embryo	Strong	Regionally restricted	1D	Expressed in the basement membrane lying between epithelial sheets of ectoderm and endoderm.
TS11: embryo ectoderm	Absent		1D
TS11: embryo endoderm	Absent		1D
TS11: extraembryonic endoderm	Absent		1D
TS11: amniotic cavity	Absent		1D	No expression in the lining cells.
TS11: ectoplacental cavity	Absent		1D	No expression in the lining cells.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	2_B Top	Expressed in the surrounding membrane.
TS15: dorsal aorta	Present	Regionally restricted	2_B Top	Expressed in the wall.
TS15: sinus venosus	Present		2_B Top
TS15: gut	Present	Regionally restricted	2_B Top	Expressed in the basement membrane.
TS15: gut epithelium	Absent		2_B Top
TS15: pericardial cavity	Absent		2_B Top
TS15: gut mesenchyme	Present	Regionally restricted	2_B Top	Expressed in the subepithelial connective tissue.
TS15: yolk sac	Present	Regionally restricted	2_B Top	Expressed in the parietal yolk sac.

Structure	Level	Pattern	Image	Note
TS15: somite	Present	Regionally restricted	2_B Middle	Expressed in the periphery.
TS15: neural tube	Present	Regionally restricted	2_B Middle	Expressed in the basement membrane.

Structure	Level	Pattern	Image	Note
TS17: somite	Present	Regionally restricted	2_B Bottom	Expressed in the surrounding membrane.
TS17: blood vessel	Present	Regionally restricted	2_B Bottom	Expressed in the intersegmental blood vessels.

Structure	Level	Pattern	Image	Note
TS15: yolk sac mesenchyme	Strong		3A	Fibrillar expression in the inner mesoderm-derived layer, including the blood vessels.
TS15: yolk sac endoderm	Absent		3A	Outer endoderm-derived layer.

Structure	Level	Pattern	Image	Note
TS15: notochord	Present	Regionally restricted	3D	Expressed in the periphery and the surrounding mesenchyme.
TS15: neural tube	Present	Regionally restricted	3D	Expressed in the basement membrane.
TS15: foregut epithelium	Present		3D

Structure	Level	Pattern	Image	Note
TS15: allantois	Present	Regionally restricted	3E	Expressed in the lining cells.
TS15: labyrinthine zone	Absent		3E
TS15: spongiotrophoblast	Absent		3E

Structure	Level	Pattern	Image	Note
TS17: endocardial cushion	Strong	Diffuse	4B Top	Fibrillar expression in the substance of the cardiac jelly between endocardium and myocardium. A tendency for concentration of staining toward the myocardium.
TS17: outflow tract	Strong	Diffuse	4B Top	Fibrillar expression in the substance of the cardiac jelly between endocardium and myocardium. A tendency for concentration of staining toward the myocardium.

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present	Regionally restricted	4B Bottom	Expressed in a perimysial/vascular pattern.
TS17: visceral pericardium	Present		4B Bottom	Expressed in epicardium.
TS17: endocardial lining	Present		4B Bottom	Expressed in endocardium.

Structure	Level	Pattern	Image	Note
TS20: trachea	Present	Regionally restricted	5B Top	Expressed in the basement membrane and underlining subepithelial connective tissue.
TS20: esophagus epithelium	Absent		5B Top
TS20: nasopharynx epithelium	Absent		5B Top
TS20: trachea epithelium	Absent		5B Top
TS20: trachea mesenchyme	Present		5B Top	Expressed in the connective tissue.
TS20: esophagus	Present	Regionally restricted	5B Top	Expressed in the basement membrane and surrounding smooth muscle.
TS20: dorsal aorta	Present	Regionally restricted	5B Top	Expressed in the wall as a multilaminar staining.

Structure	Level	Pattern	Image	Note
TS17: trachea	Present	Regionally restricted	5B Middle	Expressed in the basement membrane and the surrounding interstitial tissue, including small blood vessels.
TS17: trachea vascular element	Present	Regionally restricted	5B Middle	Expressed in small blood vessels.
TS17: trachea epithelium	Absent		5B Middle

Structure	Level	Pattern	Image	Note
TS23: lung vascular element	Present	Regionally restricted	5B Bottom	Expressed in the wall.
TS23: main bronchus	Present	Regionally restricted	5B Bottom	Expressed in the epithelial basement membrane, with sparing of epithelial cells.
TS23: main bronchus epithelium	Absent		5B Bottom

Structure	Level	Pattern	Image	Note
TS20: stomach	Present	Regionally restricted	6B Top row	Expressed in the basement membrane underlying the epithelium, the lamina propria and smooth muscle.
TS20: stomach mesenchyme	Present	Regionally restricted	6B Top row	Expressed in smooth muscle.
TS20: pancreatic duct	Strong	Regionally restricted	6B Top row	Expressed in the basement membrane.

Structure	Level	Pattern	Image	Note
TS20: gut	Present	Regionally restricted	6B Second row	Expressed in the epithelial basement membrane.
TS20: gut epithelium	Absent		6B Second row
TS20: gut mesenchyme	Present	Regionally restricted	6B Second row	Expressed in smooth muscle.

Structure	Level	Pattern	Image	Note
TS17: vitelline vein	Strong		6B Third row	Expressed in the wall.
TS17: arterial system	Strong	Regionally restricted	6B Third row	Expressed in the wall of the mesenteric artery.
TS17: pancreas primordium	Present	Regionally restricted	6B Third row	Expressed in the basement membranes of the developing acini and ducts.
TS17: venous system	Strong	Regionally restricted	6B Third row	Expressed in the wall of the portal vein.

Structure	Level	Pattern	Image	Note
TS20: liver parenchyma	Present	Regionally restricted	6B Bottom row	Expressed in a reticular pattern.
TS20: liver vascular element	Present	Regionally restricted	6B Bottom row	Expressed in the walls of hepatic veins.

Structure	Level	Pattern	Image	Note
TS20: forelimb pre-cartilage condensation	Present		7B Top row

Structure	Level	Pattern	Image	Note
TS20: dorsal aorta	Present		7B Second row
TS20: mesonephros	Present		7B Second row
TS20: vertebral cartilage condensation	Present		7B Second row
TS20: notochord	Strong	Regionally restricted	7B Second row	Fibrillar expression in the lumen of the cylinder.

Structure	Level	Pattern	Image	Note
TS24: vertebral cartilage condensation	Present	Diffuse	7B Third row	Cell and matrix staining.
TS24: nucleus pulposus	Strong	Regionally restricted	7B Third row	Fibrillar expression in the lumen of the cylinder of the notochord.

Structure	Level	Pattern	Image	Note
TS24: tongue muscle	Present		7B Bottom row
TS24: Meckel's cartilage	Present	Regionally restricted	7B Bottom row	Expressed in the peripheral in addition to diffuse matrix staining.

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted	8B Top row	Expressed in brain blood vessels and basement membrane of the meninges.
TS20: blood vessel	Present	Regionally restricted	8B Top row	Expressed in the basement membrane.
TS20: remnant of Rathke's pouch	Present	Regionally restricted	8B Top row	Expressed in the basement membrane.
TS20: face mesenchyme	Present	Regionally restricted	8B Top row	Expressed in the small blood vessels and cells within the cranial mesenchyme.

Structure	Level	Pattern	Image	Note
TS17: Rathke's pouch	Present	Regionally restricted	8B Second row	Expressed in the epithelial basement membrane.
TS17: 3rd ventricle	Present	Regionally restricted	8B Second row	Expressed in the ependyma of the infundibulum. Not expressed in the cels lining the infundibulum.

Structure	Level	Pattern	Image	Note
TS20: choroid plexus	Present	Regionally restricted	8B Third row	Expressed in the inner capillary network, and the epithelial basement membrane.

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present	Regionally restricted	8B Bottom row	Expressed in the surrounding membrane.
TS20: spinal cord	Present	Regionally restricted	8B Bottom row	Expressed in the surrounding membrane.
TS20: spinal cord vascular element	Present		8B Bottom row

Structure	Level	Pattern	Image	Note
TS28: myometrium	Strong	Regionally restricted		Expressed in the peripheral smooth muscle.
TS28: mesometrium	Strong	Regionally restricted		Expressed in the walls of the dilated blood sinuses.
TS28: decidua	Ambiguous	Regionally restricted		Little or no expression in the tissue surrounding the embryo.

Structure	Level	Pattern	Image	Note
TS28: myometrium	Present	Regionally restricted		Expressed in the inner circular layer smooth muscle. Not expressed in the dilated blood sinuses.

Structure	Level	Pattern	Image	Note
TS15: trophectoderm	Strong	Regionally restricted		Expressed in the trophoblast giant cells, but not the ectoplacental cone.
TS15: trophoblast giant cell	Strong
TS28: decidua	Strong	Regionally restricted		Expressed in the walls of vascular structures.
TS15: labyrinthine zone	Absent
TS15: spongiotrophoblast	Absent

Structure	Level	Pattern	Image	Note
TS17: axial skeleton	Present	Regionally restricted		Expressed in the condensations of the sclerotomal cells.
TS17: skeleton	Present	Regionally restricted		Expressed in the condensations of the sclerotomal cells.
TS17: cartilage condensation	Present
TS17: notochord	Present			Including the sheath.
TS17: eye mesenchyme	Present	Regionally restricted		Expressed in the extraocular muscles. Fibrillar staining in the perioptic mesenchyme, particularly the walls of capillaries, small blood vessels and the hyaloid vescular network.
TS17: paraxial mesenchyme	Present	Regionally restricted		Expressed in paraspinal muscles.
TS17: optic cup inner layer	Absent			No expression in the retina.
TS17: lens pit	Present	Regionally restricted		Expressed in the capsule, not the cells lining the vesicle.
TS17: eye surface ectoderm	Absent			Corneal ectodermal layer.

Structure	Level	Pattern	Image	Note
TS20: endocardial cushion	Strong	Diffuse		Fibrillar expression in the substance of the cardiac jelly between endocardium and myocardium. A tendency for concentration of staining toward the myocardium.
TS20: outflow tract	Strong	Diffuse		Fibrillar expression in the substance of the cardiac jelly between endocardium and myocardium. A tendency for concentration of staining toward the myocardium.
TS20: cartilage	Present
TS20: endocardial lining	Present
TS20: mesenchyme of metanephros	Present
TS20: pericardium	Present
TS20: perichondrium	Present
TS20: renal vesicle	Present
TS20: atrium cardiac muscle	Present	Regionally restricted		Interstitial expression as linear or ring pattern, consitent with perimysial and blood capillary distributions.
TS20: ventricle cardiac muscle	Present	Regionally restricted		Interstitial expression as linear or ring pattern, consitent with perimysial and blood capillary distributions.
TS20: metanephros	Present	Regionally restricted		Expressed in the basement membranes of the developing tubules and vesicles.
TS20: ureter	Present	Regionally restricted		Expressed in the walls of the renal pelvis.
TS20: renal capsule	Absent
TS20: retina	Absent
TS20: urogenital ridge	Present	Regionally restricted		Expressed in the mesothelial lining.
TS20: mesonephros	Present	Regionally restricted		Expressed in the interstitium.
TS20: mesonephric tubule	Present	Regionally restricted		Expressed in the basement membrane.
TS20: notochord	Present			Including the sheath.
TS20: skin	Present	Regionally restricted		Expressed in the epidermal basement membrane.
TS20: epidermis	Ambiguous			Relative lack of staining.
TS20: dermis	Present	Regionally restricted		Expressed in the subepithelial connective tissue.

Structure	Level	Pattern	Image	Note
TS23: Bowman's capsule of maturing renal corpuscle	Present
TS23: endocardial tissue	Present
TS23: eyelid mesenchyme	Present
TS23: glomerular basement membrane of maturing renal corpuscle	Present
TS23: glomerular mesangium of maturing glomerular tuft	Present
TS23: pericardium	Present
TS23: perichondrium	Present
TS23: atrium cardiac muscle	Present	Regionally restricted		Interstitial expression as linear or ring pattern, consitent with perimysial and blood capillary distributions.
TS23: ventricle cardiac muscle	Present	Regionally restricted		Interstitial expression as linear or ring pattern, consitent with perimysial and blood capillary distributions.
TS23: renal tubule	Present	Regionally restricted		Expressed in the basement membrane.
TS23: renal interstitium	Present	Regionally restricted		Expressed in tubular interstium.
TS23: nucleus pulposus	Strong	Regionally restricted		Expressed in the sheath.
TS23: cornea stroma	Strong	Regionally restricted		Fibrillar staining in the mesothelial layer.
TS23: skin	Present	Regionally restricted		Expressed in the epidermal basement membrane.
TS23: epidermis	Ambiguous			Relative lack of staining.
TS23: dermis	Present	Regionally restricted		Expressed in the subepithelial connective tissue.

Structure	Level	Pattern	Image	Note
TS24: Bowman's capsule of maturing renal corpuscle	Present
TS24: cartilage	Present
TS24: endocardial tissue	Present
TS24: lens capsule	Present
TS24: pericardium	Present
TS24: perichondrium	Present
TS24: tunica albuginea of testis	Present
TS24: vasculature of bladder	Present
TS24: atrium cardiac muscle	Present	Regionally restricted		Interstitial expression as linear or ring pattern, consitent with perimysial and blood capillary distributions.
TS24: ventricle cardiac muscle	Present	Regionally restricted		Interstitial expression as linear or ring pattern, consitent with perimysial and blood capillary distributions.
TS24: maturing glomerular tuft	Present	Regionally restricted		Expressed in the glomerular basement membrane, the glomerular mesangium and the walls of glomerular capillaries.
TS24: renal tubule	Present	Regionally restricted		Expressed in the tubular basement membrane and tubular interstium.
TS24: bladder	Present	Regionally restricted		Expressed in the epitheial basement membrane and the subepithelial connective tissue.
TS24: retina	Absent
TS24: urothelium of bladder	Absent
TS24: detrusor muscle of bladder	Present	Regionally restricted		Expressed in the enveloping smooth muscle.
TS24: seminiferous cord	Present	Regionally restricted		Expressed in the basement membrane.
TS24: testis mesenchyme	Present	Regionally restricted		Expressed in the interstitium.
TS24: nucleus pulposus	Present			Including the sheath.
TS24: arytenoid cartilage	Present			Elastic cartilage.
TS24: metatarsus	Present	Regionally restricted		Expressed in the ends of the bones.
TS24: extrinsic ocular muscle	Present	Diffuse		Expressed in reticular patterns.
TS24: axial muscle	Present	Regionally restricted		Reticular expression in the paraspinal muscles.
TS24: spleen	Present	Regionally restricted		Expressed in reticular patterns of vascular interstial staining.
TS24: thymus	Present	Regionally restricted		Expressed in reticular patterns of vascular interstial staining.
TS24: adenohypophysis pars anterior	Weak	Regionally restricted		Expressed in the blood vessels, not the parenchyma.
TS24: thyroid gland	Weak	Regionally restricted		Expressed in the basement membranes and the vascular interstitium.
TS24: adrenal gland	Present	Regionally restricted		Expressed in the capsule, and faint expression in the interstitium.
TS24: skin	Present	Regionally restricted		Expressed in the epidermal basement membrane.
TS24: dermis	Present	Regionally restricted		Expressed in the subepithelial connective tissue.
TS24: epidermis	Ambiguous			Relative lack of staining.