Antibody:	anti-EIIIA (3E2)
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Strong	Regionally restricted	1E	Expressed in both punctate intracellular and fibrillar intercellular patterns.
TS11: exocoelomic cavity	Strong	Regionally restricted	1E	Expressed in cells lining the exocoelomic cavity.
TS11: embryo	Strong	Regionally restricted	1E	Expressed in the basement membrane lying between epithelial sheets of ectoderm and endoderm.
TS11: embryo ectoderm	Absent		1E
TS11: amniotic cavity	Absent		1E	No expression in the lining cells.
TS11: ectoplacental cavity	Absent		1E	No expression in the lining cells.
TS11: embryo endoderm	Trace	Punctate	1E	Rare expression in the cells but not at the apices.
TS11: extraembryonic endoderm	Trace	Punctate	1E	Rare expression in the cells but not at the apices.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	2_A Top	Expressed in the surrounding membrane.
TS15: dorsal aorta	Present	Regionally restricted	2_A Top	Expressed in the wall.
TS15: sinus venosus	Present		2_A Top
TS15: gut	Present	Regionally restricted	2_A Top	Expressed in the basement membrane.
TS15: gut mesenchyme	Present	Regionally restricted	2_A Top	Expressed in the subepithelial connective tissue.
TS15: gut epithelium	Absent		2_A Top
TS15: pericardial cavity	Absent		2_A Top
TS15: yolk sac	Present	Regionally restricted	2_A Top	Expressed in the parietal yolk sac.

Structure	Level	Pattern	Image	Note
TS15: somite	Present	Regionally restricted	2_A Middle	Expressed in the periphery.
TS15: neural tube	Present	Regionally restricted	2_A Middle	Expressed in the basement membrane.

Structure	Level	Pattern	Image	Note
TS17: somite	Present	Regionally restricted	2_A Bottom	Expressed in the surrounding membrane.
TS17: blood vessel	Present	Regionally restricted	2_A Bottom	Expressed in the intersegmental blood vessels.

Structure	Level	Pattern	Image	Note
TS15: yolk sac mesenchyme	Strong		3B	Fibrillar expression in the inner mesoderm-derived layer, including the blood vessels.
TS15: yolk sac endoderm	Absent		3B	Outer endoderm-derived layer.

Structure	Level	Pattern	Image	Note
TS17: endocardial cushion	Strong	Diffuse	4A Top	Fibrillar expression in the substance of the cardiac jelly between endocardium and myocardium. A tendency for concentration of staining toward the myocardium.
TS17: outflow tract	Strong	Diffuse	4A Top	Fibrillar expression in the substance of the cardiac jelly between endocardium and myocardium. A tendency for concentration of staining toward the myocardium.

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present	Regionally restricted	4A Bottom	Expressed in a perimysial/vascular pattern.
TS17: visceral pericardium	Present		4A Bottom	Expressed in epicardium.
TS17: endocardial lining	Present		4A Bottom	Expressed in endocardium.

Structure	Level	Pattern	Image	Note
TS20: trachea	Present	Regionally restricted	5A Top	Expressed in the basement membrane and underlining subepithelial connective tissue.
TS20: esophagus epithelium	Absent		5A Top
TS20: nasopharynx epithelium	Absent		5A Top
TS20: trachea epithelium	Absent		5A Top
TS20: trachea mesenchyme	Present		5A Top	Expressed in the connective tissue.
TS20: esophagus	Present	Regionally restricted	5A Top	Expressed in the basement membrane and surrounding smooth muscle.
TS20: dorsal aorta	Present	Regionally restricted	5A Top	Expressed in the wall as a multilaminar staining.

Structure	Level	Pattern	Image	Note
TS17: trachea	Present	Regionally restricted	5A Middle	Expressed in the basement membrane and the surrounding interstitial tissue, including small blood vessels.
TS17: trachea vascular element	Present	Regionally restricted	5A Middle	Expressed in small blood vessels.
TS17: trachea epithelium	Absent		5A Middle

Structure	Level	Pattern	Image	Note
TS23: lung vascular element	Present	Regionally restricted	5A Bottom	Expressed in the wall.
TS23: main bronchus	Present	Regionally restricted	5A Bottom	Expressed in the epithelial basement membrane, with sparing of epithelial cells. Slightly less distinct staining compared with other spliced segments.
TS23: main bronchus epithelium	Absent		5A Bottom

Structure	Level	Pattern	Image	Note
TS20: stomach	Present	Regionally restricted	6A Top row	Expressed in the basement membrane underlying the epithelium, the lamina propria and smooth muscle.
TS20: stomach mesenchyme	Present	Regionally restricted	6A Top row	Expressed in smooth muscle.
TS20: pancreatic duct	Strong	Regionally restricted	6A Top row	Expressed in the basement membrane.

Structure	Level	Pattern	Image	Note
TS20: gut	Present	Regionally restricted	6A Second row	Expressed in the epithelial basement membrane.
TS20: gut epithelium	Absent		6A Second row
TS20: gut mesenchyme	Present	Regionally restricted	6A Second row	Expressed in smooth muscle.

Structure	Level	Pattern	Image	Note
TS17: vitelline vein	Strong		6A Third row	Expressed in the wall.
TS17: arterial system	Strong	Regionally restricted	6A Third row	Expressed in the wall of the mesenteric artery.
TS17: pancreas primordium	Present	Regionally restricted	6A Third row	Expressed in the basement membranes of the developing acini and ducts.
TS17: venous system	Strong	Regionally restricted	6A Third row	Expressed in the wall of the portal vein.

Structure	Level	Pattern	Image	Note
TS20: liver parenchyma	Present	Regionally restricted	6A Bottom row	Interstitial expression in a reticular pattern.
TS20: liver vascular element	Present	Regionally restricted	6A Bottom row	Expressed in the walls of hepatic veins.

Structure	Level	Pattern	Image	Note
TS20: forelimb pre-cartilage condensation	Present		7A Top row

Structure	Level	Pattern	Image	Note
TS20: notochord	Present		7A Second row	Less expression compared with other spliced segments.
TS20: vertebral cartilage condensation	Present		7A Second row	Less expression compared with other spliced segments.
TS20: dorsal aorta	Present		7A Second row	Similar expression compared with other spliced segments.
TS20: mesonephros	Present		7A Second row	Similar expression compared with other spliced segments.

Structure	Level	Pattern	Image	Note
TS24: vertebral cartilage condensation	Absent		7A Third row
TS24: nucleus pulposus	Present		7A Third row	Part of the staining in notochord is non-specific.

Structure	Level	Pattern	Image	Note
TS24: Meckel's cartilage	Absent		7A Bottom row
TS24: tongue muscle	Absent		7A Bottom row

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted	8A Top row	Expressed in brain blood vessels and basement membrane of the meninges.
TS20: blood vessel	Present	Regionally restricted	8A Top row	Expressed in the basement membrane.
TS20: remnant of Rathke's pouch	Present	Regionally restricted	8A Top row	Expressed in the basement membrane.
TS20: face mesenchyme	Present	Regionally restricted	8A Top row	Expressed in the small blood vessels and cells within the cranial mesenchyme.

Structure	Level	Pattern	Image	Note
TS17: Rathke's pouch	Present	Regionally restricted	8A Second row	Expressed in the epithelial basement membrane.
TS17: 3rd ventricle	Present	Regionally restricted	8A Second row	Expressed in the ependyma of the infundibulum. Not expressed in the cels lining the infundibulum.

Structure	Level	Pattern	Image	Note
TS20: choroid plexus	Present	Regionally restricted	8A Third row	Expressed in the inner capillary network, and the epithelial basement membrane. Weakest expression compared with other spliced segments.

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present	Regionally restricted	8A Bottom row	Expressed in the surrounding membrane.
TS20: spinal cord	Present	Regionally restricted	8A Bottom row	Expressed in the surrounding membrane.
TS20: spinal cord vascular element	Present		8A Bottom row

Structure	Level	Pattern	Image	Note
TS28: decidua	Ambiguous	Regionally restricted		Little or no expression in the tissue surrounding the embryo.

Structure	Level	Pattern	Image	Note
TS15: trophectoderm	Strong	Regionally restricted		Expressed in the trophoblast giant cells, but not the ectoplacental cone.
TS15: trophoblast giant cell	Strong
TS28: decidua	Strong	Regionally restricted		Expressed in the walls of vascular structures.
TS15: labyrinthine zone	Absent
TS15: spongiotrophoblast	Present	Regionally restricted		Interstitial expression.
TS15: notochord	Present	Regionally restricted		Expressed in the periphery and the surrounding mesenchyme.
TS15: foregut epithelium	Present
TS15: neural tube	Present	Regionally restricted		Expressed in the basement membrane.
TS28: placenta	Present	Regionally restricted		Expressed in the wall of maternal blood sinuses, but not in the placental labyrinth.

Structure	Level	Pattern	Image	Note
TS17: axial skeleton	Present	Regionally restricted		Expressed in the condensations of the sclerotomal cells.
TS17: skeleton	Present	Regionally restricted		Expressed in the condensations of the sclerotomal cells.
TS17: eye mesenchyme	Present	Regionally restricted		Expressed in the extraocular muscles. Fibrillar staining in the perioptic mesenchyme, particularly the walls of capillaries, small blood vessels and the hyaloid vescular network.
TS17: paraxial mesenchyme	Present	Regionally restricted		Expressed in paraspinal muscles.
TS17: optic cup inner layer	Absent			No expression in the retina.
TS17: lens pit	Present	Regionally restricted		Expressed in the capsule, not the cells lining the vesicle.
TS17: eye surface ectoderm	Absent			Corneal ectodermal layer.

Structure	Level	Pattern	Image	Note
TS20: endocardial cushion	Strong	Diffuse		Fibrillar expression in the substance of the cardiac jelly between endocardium and myocardium. A tendency for concentration of staining toward the myocardium.
TS20: outflow tract	Strong	Diffuse		Fibrillar expression in the substance of the cardiac jelly between endocardium and myocardium. A tendency for concentration of staining toward the myocardium.
TS20: endocardial lining	Present
TS20: mesenchyme of metanephros	Present
TS20: pericardium	Present
TS20: renal vesicle	Present
TS20: atrium cardiac muscle	Present	Regionally restricted		Interstitial expression as linear or ring pattern, consitent with perimysial and blood capillary distributions.
TS20: ventricle cardiac muscle	Present	Regionally restricted		Interstitial expression as linear or ring pattern, consitent with perimysial and blood capillary distributions.
TS20: metanephros	Present	Regionally restricted		Expressed in the basement membranes of the developing tubules and vesicles.
TS20: ureter	Present	Regionally restricted		Expressed in the walls of the renal pelvis.
TS20: renal capsule	Absent
TS20: retina	Absent
TS20: urogenital ridge	Present	Regionally restricted		Expressed in the mesothelial lining.
TS20: mesonephros	Present	Regionally restricted		Expressed in the interstitium.
TS20: mesonephric tubule	Present	Regionally restricted		Expressed in the basement membrane.
TS20: skin	Present	Regionally restricted		Expressed in the epidermal basement membrane.
TS20: dermis	Present	Regionally restricted		Expressed in the subepithelial connective tissue.
TS20: epidermis	Ambiguous			Relative lack of staining.

Structure	Level	Pattern	Image	Note
TS23: Bowman's capsule of maturing renal corpuscle	Present
TS23: endocardial tissue	Present
TS23: eyelid mesenchyme	Present
TS23: glomerular basement membrane of maturing renal corpuscle	Present
TS23: glomerular mesangium of maturing glomerular tuft	Present
TS23: pericardium	Present
TS23: atrium cardiac muscle	Present	Regionally restricted		Interstitial expression as linear or ring pattern, consitent with perimysial and blood capillary distributions.
TS23: ventricle cardiac muscle	Present	Regionally restricted		Interstitial expression as linear or ring pattern, consitent with perimysial and blood capillary distributions.
TS23: renal tubule	Present	Regionally restricted		Expressed in the basement membrane.
TS23: renal interstitium	Present	Regionally restricted		Expressed in tubular interstium.
TS23: nucleus pulposus	Trace	Regionally restricted		Very little expression in the sheath.
TS23: cornea stroma	Strong	Regionally restricted		Fibrillar staining of the mesothelial layer.
TS23: skin	Present	Regionally restricted		Expressed in the epidermal basement membrane.
TS23: dermis	Present	Regionally restricted		Expressed in the subepithelial connective tissue.
TS23: epidermis	Ambiguous			Relative lack of staining.

Structure	Level	Pattern	Image	Note
TS24: Bowman's capsule of maturing renal corpuscle	Present
TS24: endocardial tissue	Present
TS24: pericardium	Present
TS24: tunica albuginea of testis	Present
TS24: vasculature of bladder	Present
TS24: atrium cardiac muscle	Present	Regionally restricted		Interstitial expression as linear or ring pattern, consitent with perimysial and blood capillary distributions.
TS24: ventricle cardiac muscle	Present	Regionally restricted		Interstitial expression as linear or ring pattern, consitent with perimysial and blood capillary distributions.
TS24: maturing glomerular tuft	Present	Regionally restricted		Expressed in the glomerular basement membrane, the glomerular mesangium and the walls of glomerular capillaries.
TS24: renal tubule	Present	Regionally restricted		Expressed in the tubular basement membrane and tubular interstium.
TS24: bladder	Present	Regionally restricted		Expressed in the epitheial basement membrane and the subepithelial connective tissue.
TS24: frontal bone	Absent
TS24: hyoid	Absent
TS24: limb skeleton	Absent
TS24: retina	Absent
TS24: rib	Absent
TS24: urothelium of bladder	Absent
TS24: vertebra	Absent
TS24: detrusor muscle of bladder	Present	Regionally restricted		Expressed in the enveloping smooth muscle.
TS24: seminiferous cord	Present	Regionally restricted		Expressed in the basement membrane.
TS24: testis mesenchyme	Present	Regionally restricted		Expressed in the interstitium.
TS24: arytenoid cartilage	Present			Elastic cartilage.
TS24: extrinsic ocular muscle	Present	Diffuse		Expressed in reticular patterns.
TS24: axial muscle	Present	Regionally restricted		Reticular expression in the paraspinal muscles.
TS24: spleen	Present	Regionally restricted		Expressed in reticular patterns of vascular interstial staining.
TS24: thymus	Present	Regionally restricted		Expressed in reticular patterns of vascular interstial staining.
TS24: lens capsule	Ambiguous			Little or no expression.
TS24: adenohypophysis pars anterior	Trace	Regionally restricted		Faintly expressed in the blood vessels, not the parenchyma.
TS24: thyroid gland	Weak	Regionally restricted		Expressed in the basement membranes and the vascular interstitium.
TS24: adrenal gland	Present	Regionally restricted		Expressed in the capsule, and faint expression in the interstitium.
TS24: skin	Present	Regionally restricted		Expressed in the epidermal basement membrane.
TS24: dermis	Present	Regionally restricted		Expressed in the subepithelial connective tissue.
TS24: epidermis	Ambiguous			Relative lack of staining.