Gene Expression Data
Assay Details
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Assay
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Reference:
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J:193307
Wray S, et al., Spatiotemporal cell expression of luteinizing hormone-releasing hormone in the prenatal mouse: evidence for an embryonic origin in the olfactory placode. Brain Res Dev Brain Res. 1989 Apr 1;46(2):309-18
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5620651
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Gene symbol:
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Gnrh1
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Gene name:
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gonadotropin releasing hormone 1
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Antibody:
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anti-pro-LHRH antibody (LR-5)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Two antibodies against the decapeptide were mentioned, but it was not specified which one was used in the assay. It is possible MGI:3043770 was used to generate these data. Both antibodies gave similar spatiotemporal patterns.
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Results
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Specimen
3C/D:
embryonic day 12.5
(more  )
Specimen
3C/D:
(close  )
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Genetic Background:
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(C57BL/6 x CBA)F1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
3D
,
3C
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Expressed in tracks that converged at a localized point at the base of the telencephalic hemispheres. Also expressed in a few cells within the rostral telencephalon directly adjacent to this convergence point.
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Specimen
not shown:
embryonic day 13.5
(more )
Note: Results were illustrated in Fig. 4.
Specimen
not shown:
(close )
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Genetic Background:
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(C57BL/6 x CBA)F1
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Results were illustrated in Fig. 4.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: telencephalon |
Present |
Regionally restricted |
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Expressed as disperse tracks throughout the tellencephalon.
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| TS21: diencephalon |
Present |
Regionally restricted |
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Expressed within the rostral part.
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| TS21: olfactory epithelium |
Present |
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| TS21: head |
Present |
Regionally restricted |
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Expressed in tracks which originated in the olfactory epithelium and extended through the olfactory/forebrain junction into the telencephalon.
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Specimen
not shown:
embryonic day 14.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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(C57BL/6 x CBA)F1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Specimen
not shown:
embryonic day 16.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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(C57BL/6 x CBA)F1
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Specimen
not shown:
embryonic day 17.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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(C57BL/6 x CBA)F1
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: forebrain |
Present |
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Expressed in cells and fibers.Expressed in cells and fibers.Expressed in cells and fibers.
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| TS25: diencephalon |
Present |
Regionally restricted |
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Expressed in tracks throughout the diencephalon.
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| TS25: olfactory epithelium |
Absent |
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| TS25: nasal septum |
Present |
Scattered |
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Expressed in scattered cells located bilaterally, and close to the midline.
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Analysis Tools