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Gene Expression Data
Assay Details
Assay
Reference: J:213957 Howard JM, et al., Tcf3 expression marks both stem and progenitor cells in multiple epithelia. Development. 2014 Aug;141(16):3143-52
Assay type: Recombinase reporter
MGI Accession ID: MGI:5620356
Gene symbol: Bmi1
Gene name: Bmi1 polycomb ring finger oncogene
Reporter: GFP
Antibody: anti-GFP
Assay notes: Tamoxifen administered at 40 mg/ml tamoxifen in 85% corn oil/15% ethanol, 5 l/g body weight on days P21 and P22.
Results
Specimen S5A/A'': postnatal day 23; Gt(ROSA)26Sortm2(CAG-Dsred2/EGFP)Luo/Gt(ROSA)26Sor+, Bmi1tm1(cre/ERT)Mrc/Bmi1+ (more )
Note: Conditional mutant. Double labeled: green - recombinase reporter; magenta - Tcf7l1.
Structure Level Pattern Image Note
TS28: tongue epithelium Present S5A , S5A'' Recombinase reporter positive cells exhibit variable Tcf7l1 expression.
Specimen S5B/B'': postnatal day 23; Gt(ROSA)26Sortm2(CAG-Dsred2/EGFP)Luo/Gt(ROSA)26Sor+, Bmi1tm1(cre/ERT)Mrc/Bmi1+ (more )
Note: Conditional mutant. Double labeled: green - recombinase reporter; magenta - Tcf7l1.
Structure Level Pattern Image Note
TS28: tongue epithelium Present S5B , S5B'' Recombinase reporter positive cells exhibit variable Tcf3 expression.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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