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Gene Expression Data
Assay Details
Assay
Reference: J:213957 Howard JM, et al., Tcf3 expression marks both stem and progenitor cells in multiple epithelia. Development. 2014 Aug;141(16):3143-52
Assay type: Recombinase reporter
MGI Accession ID: MGI:5620309
Gene symbol: Tcf7l1
Gene name: transcription factor 7 like 1 (T cell specific, HMG box)
Reporter: GFP
Antibody: anti-GFP
Assay notes: Tamoxifen administered at 40 mg/ml tamoxifen in 85% corn oil/15% ethanol, 5 l/g body weight on days P21 and P22. Recombinase reporter expression is not detected in vehicle treated mice.
Results
Specimen 1T: postnatal adult; Gt(ROSA)26Sortm2(CAG-Dsred2/EGFP)Luo/Gt(ROSA)26Sor+, Tcf7l1tm1.1(cre/ERT2)Hng/Tcf7l1+ (more )
Note: Conditional mutant. Double labeled: green - recombinase reporter; red - Itgb4. Counterstain Hoechst 33342.
Structure Level Pattern Image Note
TS28: blood vessel Present 1T Recombinase reporter is detected in various cell types within the dermis including blood vessels.
TS28: dermis Present 1T Recombinase reporter is detected in various cell types within the dermis including blood vessels.
TS28: hair follicle Present 1T Recombinase reporter is detected in the hair follicle bulge. Lineage tracing confirms recombinase reporter expression in stem cells.
Specimen S1O: postnatal adult; Gt(ROSA)26Sortm2(CAG-Dsred2/EGFP)Luo/Gt(ROSA)26Sor+, Tcf7l1tm1.1(cre/ERT2)Hng/Tcf7l1+ (more )
Note: Conditional mutant. Counterstain Hoechst 33342.
Structure Level Pattern Image Note
TS28: esophagus basal epithelium Present S1O Lineage tracing confirms recombinase reporter expression in stem and progenitor cells.
Specimen S1Q: postnatal adult; Gt(ROSA)26Sortm2(CAG-Dsred2/EGFP)Luo/Gt(ROSA)26Sor+, Tcf7l1tm1.1(cre/ERT2)Hng/Tcf7l1+ (more )
Note: Conditional mutant. Counterstain Hoechst 33342.
Structure Level Pattern Image Note
TS28: tongue epithelium Present S1Q Expression is detected in the epithelial basal layer. Lineage tracing confirms recombinase reporter expression in stem and progenitor cells.
Specimen S1S: postnatal adult; Gt(ROSA)26Sortm2(CAG-Dsred2/EGFP)Luo/Gt(ROSA)26Sor+, Tcf7l1tm1.1(cre/ERT2)Hng/Tcf7l1+ (more )
Note: Conditional mutant. Counterstain Hoechst 33342.
Structure Level Pattern Image Note
TS28: autopod skin Present S1S Expression is detected in the epithelial basal layer. Lineage tracing confirms recombinase reporter expression in stem and progenitor cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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