Probe:	Periostin cDNA1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted	2A	Expressed in mesenchymal decidual cells in the maternal region of future placenta.
TS14: ectoplacental cone	Absent		2A
TS14: labyrinthine zone	Absent		2A

Structure	Level	Pattern	Image	Note
TS15: endocardial cushion	Present	Regionally restricted	2E	Expressed in the mesenechyme of the cardiac atrioventricular cushions.
TS15: chorionic plate	Present		2E
TS15: trunk mesenchyme	Present	Regionally restricted	2E	Expressed at lower levels in the mesenchyme surrounding the hindgut and cardinal veins.
TS15: yolk sac	Present	Regionally restricted	2E	Expressed in the blood islands.

Structure	Level	Pattern	Image	Note
TS16: endocardial cushion	Present	Regionally restricted	3A	Expressed in the endocardial cushion mesenchyme of the atrioventricular canal and in outflow tract cushions cells.

Structure	Level	Pattern	Image	Note
TS17: endocardial cushion	Strong	Regionally restricted	3E	Expressed in the mesenechyme of the cardiac atrioventricular cushions.
TS17: endocardial lining	Absent		3E

Structure	Level	Pattern	Image	Note
TS20: endocardial cushion	Strong	Regionally restricted	3I	Expressed in the endocardial cushion mesenchyme of the atrioventricular canal and in outflow tract cushions cells.
TS20: spinal nerve	Present		3I	Expressed in the peripheral nerves.
TS20: pericardium	Present	Regionally restricted	3I	Expressed in the pericardium overlying the ventricle.
TS20: atrioventricular endocardial cushion	Strong	Regionally restricted	3I	Expressed in the endocardial cushion mesenchyme of the atrioventricular canal.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Absent		4A
TS22: meninges	Present	Regionally restricted	4A	Expressed in tissues surrounding the spinal cord.
TS22: periosteum	Present	Regionally restricted	4A	Expressed in the periostium around the skeleton.

Structure	Level	Pattern	Image	Note
TS22: endocardial cushion	Strong		4D
TS22: ascending aorta	Present		4D	Proximal aorta.
TS22: heart mesentery	Present	Single cells	4D	Expressed in isolated cardiac fibroblasts around the outflow tract cushions.
TS22: trunk mesenchyme	Present	Regionally restricted	4D	Expressed in the mesenchyme adjacent to the ductus arteriosus.

Structure	Level	Pattern	Image	Note
TS22: mitral valve	Strong	Regionally restricted	4G	Expressed in the mesenchyme in all leaflets.
TS22: tricuspid valve	Strong	Regionally restricted	4G	Expressed in the mesenchyme in all leaflets.

Structure	Level	Pattern	Image	Note
TS22: outflow tract	Present		4J' , 4J
TS22: periosteum	Present	Regionally restricted	4J' , 4J	Expressed in the periostium surrounding the rib.

Structure	Level	Pattern	Image	Note
TS28: aortic valve	Strong	Regionally restricted	5A	Expressed in leaflet fibrous mesenchyme, chordae tendi and the cardiac fibroblasts that anchor the valves within the papillary muscles.

Structure	Level	Pattern	Image	Note
TS28: lung	Present	Widespread	5A inset

Structure	Level	Pattern	Image	Note
TS27: heart valve	Present	Regionally restricted		Expressed in the fibrous mesenchyme and the valvular suspensory apparatus.