Gtf2i Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:163633 Fijalkowska I, et al., Expression of the transcription factor, TFII-I, during post-implantation mouse embryonic development. BMC Res Notes. 2010;3:203
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5619503
Gene symbol:	Gtf2i
Gene name:	general transcription factor II I

Antibody:	TFII-I antibody (Ab 3588)
Detection system:	Biotinylated secondary antibody/[Strept]avidin

Results

1A ©

Specimen 1A: embryonic day 8.0 (more

)

Structure	Level	Image	Note
TS12: amnion	Strong	1A
TS12: trophectoderm	Strong	1A
TS28: placenta	Strong	1A
TS12: embryo mesenchyme	Present	1A	Cytoplasmic staining.
TS12: surface ectoderm	Present	1A	Cytoplasmic staining.

1B ©

Specimen 1B: embryonic day 8.0 (more

)

Structure	Level	Image	Note
TS12: embryo mesenchyme	Present	1B	Cytoplasmic staining.
TS12: neural ectoderm	Present	1B	Cytoplasmic staining.
TS12: amnion	Present	1B

1C ©

Specimen 1C: embryonic day 8.0 (more

)

Structure	Level	Image
TS12: amnion	Present	1C
TS12: trophectoderm	Present	1C
TS12: yolk sac	Present	1C

1D ©

Specimen 1D: embryonic day 8.0 (more

)

Structure	Level	Pattern	Image	Note
TS12: future brain	Present		1D	Cytoplasmic staining.
TS12: head mesenchyme	Present		1D	Cytoplasmic staining.

2A ©

Specimen 2A: embryonic day 9.0 (more

)

Structure	Level	Pattern	Image	Note
TS14: future brain	Very strong		2A	Nuclear staining, some diffuse cytoplasmic staining.
TS14: embryo	Present	Regionally restricted	2A

2B ©

Specimen 2B: embryonic day 9.0 (more

)

Structure	Level	Pattern	Image	Note
TS14: optic vesicle	Present	Regionally restricted	2B	Expression is in the inner and outer retinal layer.
TS14: eye	Present	Regionally restricted	2B	Expression is in the lens primordium.

2C ©

Specimen 2C: embryonic day 9.0 (more

)

Structure	Level	Pattern	Image	Note
TS14: neural tube	Strong		2C	Nuclear staining.

2D ©

2D inset ©

Specimen 2D: embryonic day 9.0 (more

)
Note: Inset shows a higher magnification.

Structure	Level	Pattern	Image	Note
TS14: future brain	Strong		2D , 2D inset	Nuclear staining, some diffuse cytoplasmic staining.

2E ©

Specimen 2E: embryonic day 12.0 (more

)

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		2E

2G ©

Specimen 2G: embryonic day 12.0 (more

)

Structure	Level	Pattern	Image	Note
TS20: nasopharynx	Present		2G	Cytoplasmic staining.

3A ©

Specimen 3A: embryonic day 11.0 (more

)

Structure	Level	Pattern	Image	Note
TS18: liver	Weak	Diffuse	3A	Cytoplasmic staining.

3B ©

Specimen 3B: embryonic day 15.0 (more

)

Structure	Level	Pattern	Image
TS23: diaphragm	Present		3B
TS23: lung	Present		3B
TS23: liver	Weak	Diffuse	3B

4A ©

Specimen 4A: embryonic day 12.0 (more

)

Structure	Level	Pattern	Image	Note
TS20: heart	Strong	Uniform	4A	Cytoplasmic staining.

4B ©

Specimen 4B: embryonic day 12.0 (more

)

Structure	Level	Pattern	Image	Note
TS20: lung epithelium	Strong		4B
TS20: lung	Present		4B
TS20: lung vascular element	Present	Regionally restricted	4B	Expression is in endothelial cells.

4C ©

Specimen 4C: embryonic day 16.0 (more

)

Structure	Level	Pattern	Image	Note
TS24: lung	Strong		4C	Nuclear staining in developing air sacs.
TS24: lung epithelium	Present		4C	Nuclear staining.
TS24: lung vascular element	Present	Regionally restricted	4C	Nuclear expression in endothelial cells.

4E ©

Specimen 4E: embryonic day 15.0 (more

)

Structure	Level	Pattern	Image	Note
TS23: lung	Present		4E	Expression is in developing air sacs.
TS23: lung vascular element	Present	Regionally restricted	4E	Expression is in endothelial cells.

5A ©

Specimen 5A: embryonic day 15.0 (more

)

Structure	Level	Pattern	Image	Note
TS23: epidermis	Strong		5A
TS23: dermis	Strong		5A	Expression is both nuclear and cytoplasmic in the superficial dermal layers in smooth muscle and in underlying mesenchyme and vessels.
TS23: blood vessel	Present	Regionally restricted	5A	Expression is in endothelial cells in the skin.

5B ©

Specimen 5B: embryonic day 15.0 (more

)

Structure	Level	Pattern	Image	Note
TS23: submandibular gland primordium	Strong		5B

5C ©

Specimen 5C: embryonic day 16.0 (more

)

Structure	Level	Pattern	Image	Note
TS24: epidermis	Strong		5C
TS24: dermis	Strong		5C	Expression is both nuclear and cytoplasmic in the superficial dermal layers, in smooth muscle and in underlying mesenchyme and vessels.
TS24: blood vessel	Present	Regionally restricted	5C	Expression is in endothelial cells in the skin.

5D ©

5D inset ©

Specimen 5D: embryonic day 16.0 (more

)
Note: Inset shows a higher magnification.

Structure	Level	Pattern	Image	Note
TS24: intestine	Strong	Regionally restricted	5D , 5D inset	Expression is both nuclear and cytoplasmic in the villi.

Specimen not shown E11: embryonic day 11.0 (more

)

Structure	Level	Pattern
TS18: lung epithelium	Strong
TS18: heart	Strong	Uniform
TS18: blood vessel	Present
TS18: surface ectoderm	Present

Specimen not shown A: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: alveolar sac	Present
TS28: lung vascular element	Present

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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