Antibody:	HB-78 (MMA)
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS13: otic placode epithelium	Strong		1A

Structure	Level	Pattern	Image	Note
TS16: otocyst epithelium	Present	Uniform	1B	Homogenous expression.

Structure	Level	Pattern	Image	Note
TS16: otocyst epithelium	Present	Regionally restricted	1C	Positive and -negative areas begin to segregate.

Structure	Level	Pattern	Image	Note
TS19: utricle epithelium	Present	Regionally restricted	1D	Expressed in several segments of the epithelial ridge, filigree fibres on the epithelial cell surface, and precipitate-like material covering the luminal surface.

Structure	Level	Pattern	Image	Note
TS20: inner ear epithelium	Present	Regionally restricted	1E	Expressed in the lumen of the channel and the forming maculae and cochlear duct. The immunoreactive cells invaginate from the lumen into the epithelial layer.
TS20: vestibular component epithelium	Strong	Regionally restricted	1E	Intensely stained cells extending from the luminal surface to the basal membrane in the non-sensory area.
TS20: utricle epithelium	Present	Regionally restricted	1E	Expressed in the future receptor cell layer of maculae luminally.
TS20: cochlear duct epithelium	Present	Regionally restricted	1E	The immunoreactive cells invaginate from the lumen into the epithelial layer.

Structure	Level	Pattern	Image	Note
TS22: cochlear duct epithelium	Present	Scattered	1F	Intracellularly labelled.
TS22: endolymphatic sac epithelium	Present	Scattered	1F	Intracellularly labelled.
TS22: saccule epithelium	Present	Scattered	1F	Intracellularly labelled.
TS22: semicircular canal epithelium	Present	Scattered	1F	Intracellularly labelled.

Structure	Level	Pattern	Image	Note
TS21: cochlear duct epithelium	Present	Regionally restricted	2A	Expressed in the lumen and cells growing into the epithelial layer.

Structure	Level	Pattern	Image	Note
TS22: cochlear duct epithelium	Present	Regionally restricted	2B	Expressed in the rostral wall and especially the caudal wall (the greater and lesser epithelial ridge), and the tectorial membrane.

Structure	Level	Pattern	Image	Note
TS24: cochlear duct epithelium	Strong	Regionally restricted	2C	Positive stained tectorial membrane covering the unstained greater epithelial ridge is associated with the strong positive cell bundle in the caudal/rostral connection.

Structure	Level	Pattern	Image	Note
TS25: organ of Corti	Present	Regionally restricted	2D	Expressed in the supporting cells adjacent to the inner hair cell and the outer surface of the inner and outer hair cells. The future dental cells of spiral limbus are negative.

Structure	Level	Pattern	Image	Note
TS26: organ of Corti	Present	Regionally restricted	2E	Expressed in the supporting cells adjacent to the inner hair cell and the outer surface of the inner and outer hair cells. The region of the stria vascularis is becoming outlined. The intensity of the staining decreases.

Structure	Level	Pattern	Image	Note
TS26: organ of Corti	Strong	Regionally restricted	2F	Expressed in the supporting cells adjacent to the inner hair cell, the tectorial membrane, epithelial layer of the Reissner's membrane, the spiral prominentia and the basal layer of the stria vascularis.

Structure	Level	Pattern	Image	Note
TS23: utricle epithelium	Strong	Regionally restricted	3A	Heavily stained cristae ampullares show more uniform labelling of their cell surface. The maculae of the saccule and the utricle contain restricted negative and positive cell groups, with expression in the cell surface.
TS23: saccule epithelium	Strong	Regionally restricted	3A	Strong cell surface labelling in non-sensory areas.

Structure	Level	Pattern	Image	Note
TS25: utricle epithelium	Strong	Regionally restricted	3B	Heavily stained cristae ampullares show more uniform labelling of their cell surface. The maculae of the saccule and the utricle contain restricted negative and positive cell groups, with expression in the cell surface.
TS25: saccule epithelium	Strong	Regionally restricted	3B	Strong cell surface labelling in non-sensory areas.

Structure	Level	Pattern	Image	Note
TS26: utricle epithelium	Present	Regionally restricted	3C	Distinct staining pattern between forming receptor (intracellular staining) and supporting cells (web-like staining). Strong cell surface labelling in non-sensory areas.
TS26: saccule epithelium	Strong	Regionally restricted	3C	Strong cell surface labelling in non-sensory areas.

Structure	Level	Pattern	Image	Note
TS26: utricle epithelium	Present	Regionally restricted	3D	Expressed in the sensory area of maculae and cristae ampulares. The membranes of otoconia and cupula are stained. Strong cell surface labelling in non-sensory areas.
TS26: saccule epithelium	Strong	Regionally restricted	3D	Strong cell surface labelling in non-sensory areas.

Structure	Level	Pattern	Image	Note
TS20: endolymphatic sac	Present	Scattered	3E

Structure	Level	Pattern	Image	Note
TS24: endolymphatic sac	Strong	Scattered	3F	The precipitate in the sac lumen shows intense immunostaining.

Structure	Level	Pattern	Image	Note
TS26: endolymphatic sac	Present	Scattered	3G