Antibody:	Anti-Lyn
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present

Structure	Level	Pattern	Image	Note
TS13: future spinal cord neural plate	Present
TS13: neural tube	Present
TS13: neural ectoderm	Present	Regionally restricted		Expression was detected in the neural tube when it was partially closed.

Structure	Level	Pattern	Image	Note
TS17: brain	Strong	Regionally restricted		Expression was mainly distributed on the margin of the primordial plexiform layer.
TS17: future spinal cord	Strong	Regionally restricted		Expression was mainly distributed on the margin of the primordial plexiform layer.
TS17: forebrain	Present	Regionally restricted		Expression was dorsal.
TS17: midbrain	Present	Regionally restricted		Expression was dorsal.

Structure	Level	Pattern	Image	Note
TS19: brain	Present	Regionally restricted		Expression was mainly distributed on the margin of the primordial plexiform layer.
TS19: future spinal cord	Present	Regionally restricted		Expression was mainly distributed on the margin of the primordial plexiform layer.
TS19: forebrain	Present	Regionally restricted		Expression was dorsal.
TS19: midbrain	Present	Regionally restricted		Expression was dorsal.

Structure	Level	Pattern	Image	Note
TS20: forebrain ventricular layer	Weak
TS20: midbrain roof plate	Weak
TS20: midbrain ventricular layer	Weak
TS20: forebrain	Weak	Regionally restricted		Expression was low in the roof of the forebrain.
TS20: vagus ganglion	Present
TS20: vagal nerve trunk	Present	Regionally restricted		Expression was detected in nerve bundles around the cardiovascular system and respiratory tract.

Structure	Level	Pattern	Image	Note
TS20: cortical plate	Strong	Regionally restricted	1F	Strongly expressing nerve bundles were noted.

Structure	Level	Pattern	Image	Note
TS17: hindbrain marginal layer	Present		2A
TS17: trigeminal ganglion	Present		2A

Structure	Level	Pattern	Image	Note
TS20: trigeminal ganglion	Present		2B
TS20: trigeminal nerve mandibular division	Present		2B
TS20: trigeminal nerve maxillary division	Present		2B
TS20: trigeminal nerve ophthalmic division	Present		2B

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Strong		3B
TS20: spinal cord	Strong		3B
TS20: spinal nerve	Strong		3B

Structure	Level	Pattern	Image	Note
TS24: retina	Moderate	Regionally restricted	4C	Expression was restricted to the inner retina.