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Gene Expression Data
Assay Details
Assay
Reference: J:86196 Bell SM, et al., Sp8 is crucial for limb outgrowth and neuropore closure. Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12195-200
Assay type: RNA in situ
MGI Accession ID: MGI:5617516
Gene symbol: En1
Gene name: engrailed 1
Probe: En1 probe21
Probe preparation: Antisense RNA
Results
Specimen 3C: embryonic day 9.75 (more )
Structure Level Pattern Image Note
TS15: forelimb bud ectoderm Present Regionally restricted 3C
Specimen 3D: embryonic day 9.75; Sp8tm1Smb/Sp8tm1Smb (more )
Structure Level Pattern Image Note
TS15: forelimb bud ectoderm Present Regionally restricted 3D
Specimen 3I: embryonic day 10.0 (more )
Structure Level Pattern Image Note
TS16: forelimb bud apical ectodermal ridge Present 3I
TS16: forelimb bud ectoderm Present Regionally restricted 3I Expression is in the proximo-ventral ectoderm.
Specimen 3J: embryonic day 10.0; Sp8tm1Smb/Sp8tm1Smb (more )
Structure Level Pattern Image Note
TS16: forelimb bud ectoderm Present Regionally restricted 3J Expression is in the proximo-ventral ectoderm, absent in the disto-ventral AER.
TS16: forelimb bud apical ectodermal ridge Absent 3J
Specimen 3O: embryonic day 10.5-11.0 (more )
Structure Level Pattern Image Note
TS17: forelimb bud apical ectodermal ridge Present 3O
TS17: hindlimb bud apical ectodermal ridge Present 3O
TS17: somite Present 3O
Specimen 3P: embryonic day 10.5-11.0; Sp8tm1Smb/Sp8tm1Smb (more )
Structure Level Pattern Image Note
TS17: forelimb bud apical ectodermal ridge Absent 3P
TS17: hindlimb bud ectoderm Absent 3P Absent in the distal tips.
TS17: somite Present Regionally restricted 3P Expression is in the somites anterior to hindlimb since somite formation posterior to hindlimbs is absent in this mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory