Gli1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:135300 Babbs C, et al., Polydactyly in the mouse mutant Doublefoot involves altered Gli3 processing and is caused by a large deletion in cis to Indian hedgehog. Mech Dev. 2008 May-Jun;125(5-6):517-26
Assay type:	RNA in situ
MGI Accession ID:	MGI:5616627
Gene symbol:	Gli1
Gene name:	GLI-Kruppel family member GLI1

Probe:	Gli1 probe22
Probe preparation:	Antisense labelled with digoxigenin RNA

Results

4_Gli1 wt ©

Specimen 4 wt: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	4_Gli1 wt

4_Gli1 Dbf/+ ©

Specimen 4 Dbf/+: embryonic day 10.5; Dbf/Dbf⁺ (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	4_Gli1 Dbf/+	The expression domain is expanded anteriorly in this mutant.

Specimen 4 Shh-/-: embryonic day 10.5; Shh^tm1Chg/Shh^tm1Chg (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Absent		4_Gli1 Shh-/-

Specimen 4 Shh-/-;Dbf/+: embryonic day 10.5; Dbf/Dbf⁺, Shh^tm1Chg/Shh^tm1Chg (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	4_Gli1 Shh-/-;Dbf/+	The expression domain is broader in the proximal mesenchyme in this mutant.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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