Gene Expression Data
Assay Details
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Assay
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Reference:
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J:135300
Babbs C, et al., Polydactyly in the mouse mutant Doublefoot involves altered Gli3 processing and is caused by a large deletion in cis to Indian hedgehog. Mech Dev. 2008 May-Jun;125(5-6):517-26
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5616601
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Gene symbol:
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Ihh
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Gene name:
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Indian hedgehog
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Probe:
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Ihh probe19
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
3C:
embryonic day 10.5; Dbf/Dbf +
(more  )
Specimen
3C:
(close  )
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Genetic Background:
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involves: 101/H * 129S1/Sv * 129X1/SvJ * C3H/HeH * C57BL/6J
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Mutant Allele(s):
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Dbf/Dbf+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
3C
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Expression is in the distal mesenchyme, absent in the zone of polarizing activity.
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Specimen
3D:
embryonic day 10.5; Dbf/Dbf +
(more )
Note: Double labeled: blue - Ihh; blue - Shh (assay MGI:5616593).
Specimen
3D:
(close )
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Genetic Background:
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involves: 101/H * 129S1/Sv * 129X1/SvJ * C3H/HeH * C57BL/6J
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Mutant Allele(s):
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Dbf/Dbf+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: blue - Ihh; blue - Shh (assay MGI:5616593).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
3D
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Expression is in the distal mesenchyme, absent in the zone of polarizing activity.
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Specimen
3E:
(close )
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Genetic Background:
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involves: 101/H * 129S1/Sv * 129X1/SvJ * C3H/HeH * C57BL/6J
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Mutant Allele(s):
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Dbf/Dbf+
Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
3E
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In this mutant the expression domain extends throughout the distal mesenchyme.
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Specimen
3H:
embryonic day 11.5; Dbf/Dbf +
(more )
Specimen
3H:
(close )
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Genetic Background:
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involves: 101/H * 129S1/Sv * 129X1/SvJ * C3H/HeH * C57BL/6J
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Mutant Allele(s):
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Dbf/Dbf+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3I:
(close )
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Genetic Background:
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involves: 101/H * 129S1/Sv * 129X1/SvJ * C3H/HeH * C57BL/6J
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Mutant Allele(s):
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Dbf/Dbf+
Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Analysis Tools
