Gene Expression Data
Assay Details
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Assay
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Reference:
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J:111998
Reinholdt LG, et al., The mouse gcd2 mutation causes primordial germ cell depletion. Mech Dev. 2006 Jul;123(7):559-69
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5613934
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Gene symbol:
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Gcna
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Gene name:
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germ cell nuclear acidic protein
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Antibody:
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anti-GCNA
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Detection system:
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Secondary antibody coupled to Cy3
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Results
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Specimen
3A:
postnatal day 1
(more  )
Specimen
3A:
(close  )
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Genetic Background:
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involves: 129S1/Sv * CAST/EiJ
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Age:
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postnatal day 1
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3B:
(close )
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Genetic Background:
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CAST.129S1(B6)-Kif18agcd2/JcsJ
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Mutant Allele(s):
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Kif18agcd2/Kif18agcd2
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Age:
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postnatal day 1
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: germ cell of ovary |
Trace |
Single cells |
3B
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The severity of germ cell depletion in this mutant varied per individual.
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Specimen
3C:
postnatal day 1
(more )
Specimen
3C:
(close )
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Genetic Background:
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involves: 129S1/Sv * CAST/EiJ
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Age:
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postnatal day 1
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3D:
(close )
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Genetic Background:
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CAST.129S1(B6)-Kif18agcd2/JcsJ
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Mutant Allele(s):
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Kif18agcd2/Kif18agcd2
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Age:
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postnatal day 1
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: germ cell of testis |
Present |
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3D
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The severity of germ cell depletion in this mutant varied per individual.
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Specimen
4G:
embryonic day 15.5
(more )
Note: Double labeled: green - Gcna; red - laminin.
Specimen
4G:
(close )
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Genetic Background:
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involves: 129S1/Sv * CAST/EiJ
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Age:
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embryonic day 15.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Gcna; red - laminin.
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Specimen
4H:
(close )
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Genetic Background:
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CAST.129S1(B6)-Kif18agcd2/JcsJ
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Mutant Allele(s):
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Kif18agcd2/Kif18agcd2
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Age:
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embryonic day 15.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: germ cell of testis |
Trace |
Single cells |
4H
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The severity of germ cell depletion in this mutant varied per individual.
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Analysis Tools