Gene Expression Data
Assay Details
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Assay
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Reference:
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J:35177
Fong GH, et al., Regulation of flt-1 expression during mouse embryogenesis suggests a role in the establishment of vascular endothelium. Dev Dyn. 1996 Sep;207(1):1-10
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5613016
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Gene symbol:
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Flt1
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Gene name:
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FMS-like tyrosine kinase 1
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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Fixative was 0.2% glutaraldehyde. For paraffin sections the specimens were postfixed in 3.7% formaldehyde prior to emdedding.
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Results
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Specimen
2B:
embryonic day 8.5; Flt1tm1Jrt/Flt1 +
(more  )
Specimen
2B:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Flt1tm1Jrt/Flt1+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
2B inset:
embryonic day 8.5; Flt1tm1Jrt/Flt1 +
(more )
Specimen
2B inset:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Flt1tm1Jrt/Flt1+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
2C:
embryonic day 11.5; Flt1tm1Jrt/Flt1 +
(more )
Specimen
2C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Flt1tm1Jrt/Flt1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
2D:
embryonic day 11.5; Flt1tm1Jrt/Flt1 +
(more )
Specimen
2D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Flt1tm1Jrt/Flt1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: arterial system |
Present |
Regionally restricted |
2D
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Expression is in intersomitic arteries.
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Specimen
2E:
embryonic day 11.5; Flt1tm1Jrt/Flt1 +
(more )
Specimen
2E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Flt1tm1Jrt/Flt1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: yolk sac |
Present |
Regionally restricted |
2E
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Expression is in endothelial cells.
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Specimen
3A:
embryonic day 6.5; Flt1tm1Jrt/Flt1 +
(more )
Note: Early streak stage.
Specimen
3A:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Flt1tm1Jrt/Flt1+
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Early streak stage.
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Specimen
3B:
embryonic day 7.5; Flt1tm1Jrt/Flt1 +
(more )
Note: Mid streak stage.
Specimen
3B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Flt1tm1Jrt/Flt1+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Mid streak stage.
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Specimen
3D:
embryonic day 7.5; Flt1tm1Jrt/Flt1 +
(more )
Specimen
3D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Flt1tm1Jrt/Flt1+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Specimen
3E:
embryonic day 7.5; Flt1tm1Jrt/Flt1 +
(more )
Specimen
3E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Flt1tm1Jrt/Flt1+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Specimen
not shown:
embryonic day 7.5-8.0; Flt1tm1Jrt/Flt1 +
(more )
Note: Late streak/early headfold stage.
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Flt1tm1Jrt/Flt1+
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Age:
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embryonic day 7.5-8.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Note:
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Late streak/early headfold stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo mesoderm |
Present |
Regionally restricted |
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Expression is in the proximal-lateral mesoderm and the paired strips of mesoderm cells along the future location of the dorsal aortae.
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Specimen
4A:
embryonic day 8.0; Flt1tm1Jrt/Flt1 +
(more )
Specimen
4A:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Flt1tm1Jrt/Flt1+
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
4B:
embryonic day 8.5; Flt1tm1Jrt/Flt1 +
(more )
Specimen
4B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Flt1tm1Jrt/Flt1+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
4C:
embryonic day 9.5; Flt1tm1Jrt/Flt1 +
(more )
Specimen
4C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Flt1tm1Jrt/Flt1+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
4D:
embryonic day 15.5; Flt1tm1Jrt/Flt1 +
(more )
Specimen
4D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Flt1tm1Jrt/Flt1+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
5A:
embryonic day 13.5; Flt1tm1Jrt/Flt1 +
(more )
Specimen
5A:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Flt1tm1Jrt/Flt1+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
5B:
embryonic day 13.5; Flt1tm1Jrt/Flt1 +
(more )
Specimen
5B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Flt1tm1Jrt/Flt1+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
5C:
embryonic day 15.5; Flt1tm1Jrt/Flt1 +
(more )
Specimen
5C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Flt1tm1Jrt/Flt1+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: brain meninges |
Present |
Regionally restricted |
5C
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Expression is in most endothelial cells of the leptomeninges.
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| TS23: brain vascular element |
Present |
Regionally restricted |
5C
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Expression is in most endothelial cells of the leptomeninges.
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Specimen
5D:
embryonic day 15.5; Flt1tm1Jrt/Flt1 +
(more )
Specimen
5D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Flt1tm1Jrt/Flt1+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: liver vascular element |
Present |
Regionally restricted |
5D
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Expression is in numerous endothelial cells in the outer zone, absent in in the endothelial cells in the interior of the liver lobule.
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Specimen
6A:
embryonic day 7.5; Flt1tm1Jrt/Flt1 +
(more )
Specimen
6A:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Flt1tm1Jrt/Flt1+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Specimen
6B:
embryonic day 8.0; Flt1tm1Jrt/Flt1 +
(more )
Specimen
6B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Flt1tm1Jrt/Flt1+
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: blood island |
Present |
Regionally restricted |
6B
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Expression is weaker in cells in the interior of the blood island.
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Specimen
6C:
embryonic day 8.5; Flt1tm1Jrt/Flt1 +
(more )
Specimen
6C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Flt1tm1Jrt/Flt1+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: yolk sac |
Strong |
Regionally restricted |
6C
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Expression is in endothelial cells.
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Specimen
6D:
embryonic day 11.5; Flt1tm1Jrt/Flt1 +
(more )
Specimen
6D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * CD-1
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Mutant Allele(s):
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Flt1tm1Jrt/Flt1+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: yolk sac |
Present |
Regionally restricted |
6D
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Expression is in endothelial cells.
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