Antibody:	anti-N-CAM
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS13: cardiac muscle tissue	Strong		3A/D
TS13: future prosencephalon	Moderate	Regionally restricted	3A/D	Expressed in the neuroectoderm such as the telocoel.
TS13: head mesenchyme	Weak		3A/D
TS13: endocardial tube	Absent		3A/D

Structure	Level	Pattern	Image	Note
TS13: somite	Strong		3B/E
TS13: surface ectoderm	Absent		3B/E

Structure	Level	Pattern	Image	Note
TS13: trunk unsegmented mesenchyme	Strong		3C/F	Expressed in the segmental plate.
TS13: foregut diverticulum endoderm	Absent		3C/F
TS13: hindgut diverticulum endoderm	Absent		3C/F

Structure	Level	Pattern	Image	Note
TS15: future brain	Strong		4A/D	Expressed in all main brain regions.
TS15: neural tube	Strong		4A/D

Structure	Level	Pattern	Image	Note
TS15: somite	Strong	Non-Uniform	4B/E	Expression in the dermamyotome is higher than the sclerotome. Younger, epithelial somites were more strongly positive than differentiating somites.
TS15: dermomyotome	Strong		4B/E
TS15: sclerotome	Strong		4B/E

Structure	Level	Pattern	Image	Note
TS14: future spinal cord neural plate	Strong	Regionally restricted	4C/F	Expressed in the future spinal cord, as well as in the neural plate in the posterior part of the embryo.
TS14: foregut epithelium	Absent		4C/F
TS14: hindgut diverticulum epithelium	Absent		4C/F

Structure	Level	Pattern	Image	Note
TS14: heart	Strong
TS14: optic vesicle	Weak
TS14: otic placode	Weak

Structure	Level	Pattern	Image	Note
TS15: heart	Strong
TS15: optic vesicle	Weak
TS15: otocyst	Weak