Probe:	Klf4 cDNA3
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS16: head mesenchyme	Present	Regionally restricted	2A	Expression seen in mesenchyme adjacent to the fourth arch artery.

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Present	Regionally restricted	2B	Expression seen in mesenchyme adjacent to the fourth arch artery.
TS17: 1st branchial arch	Present	Regionally restricted	2B	Expression seen in the medial part.

Structure	Level	Pattern	Image	Note
TS18: head mesenchyme	Present	Regionally restricted	2C	Expression seen in mesenchyme adjacent to the fourth arch artery.
TS18: head	Present	Regionally restricted	2C	Expression seen near the developing eye and the aboral side of the 2nd and 3rd branchial arches.
TS18: apical ectodermal ridge	Present		2C

Structure	Level	Pattern	Image	Note
TS19: head mesenchyme	Present	Regionally restricted	2D	Expression seen in mesenchyme adjacent to the fourth arch artery.
TS19: head	Present	Regionally restricted	2D	Expression seen near the developing eye and the aboral side of the 2nd and 3rd branchial arches.
TS19: vertebral pre-cartilage condensation	Present		2D	Expression seen in the mesenchyme of the developing vertebrae.
TS19: apical ectodermal ridge	Present		2D
TS19: trunk	Present	Regionally restricted	2D	Expression seen in the abdominal region of the ventral body.
TS19: nasal process mesenchyme	Present		2D	Expression seen in the mesenchyme of the nasal pits.

Structure	Level	Pattern	Image	Note
TS19: vertebral pre-cartilage condensation	Present		2E	Expression seen in the mesenchyme of the developing vertebrae.
TS19: apical ectodermal ridge	Present		2E

Structure	Level	Pattern	Image	Note
TS19: head mesenchyme	Present	Regionally restricted	2F	Expression seen in mesenchyme adjacent to the fourth arch artery.
TS19: head	Present	Regionally restricted	2F	Expression seen near the developing eye and the aboral side of the 2nd and 3rd branchial arches.
TS19: vertebral pre-cartilage condensation	Present		2F	Expression seen in the mesenchyme of the developing vertebrae.
TS19: apical ectodermal ridge	Present		2F
TS19: trunk	Present	Regionally restricted	2F	Expression seen in the abdominal region of the ventral body.
TS19: nasal process mesenchyme	Present	Regionally restricted	2F	Expression seen in cells surrounding the nasal pits.

Structure	Level	Pattern	Image	Note
TS20: limb epithelium	Present	Regionally restricted	2G	Expression is seen in the apical ectodermal ridge.
TS20: trunk	Present	Regionally restricted	2G	Expression seen in the abdominal region of the ventral body.
TS20: head	Present	Regionally restricted	2G	Diffuse expression seen craniofacial region.
TS20: vibrissa	Present		2G

Structure	Level	Pattern	Image	Note
TS20: limb epithelium	Present	Regionally restricted	2H	Expression is seen in the apical ectodermal ridge.
TS20: trunk	Present	Regionally restricted	2H	Expression seen in the abdominal region of the ventral body.
TS20: head	Present	Regionally restricted	2H	Diffuse expression seen craniofacial region.
TS20: vibrissa	Present		2H

Structure	Level	Pattern	Image	Note
TS21: trunk	Present	Regionally restricted	2I	Expression seen in the abdominal region of the ventral body.
TS21: vibrissa	Present		2I
TS21: epidermis	Present	Diffuse	2I

Structure	Level	Pattern	Image	Note
TS19: head mesenchyme	Present		3B , 3A	Expression is not seen in the epithelial cells of the head.

Structure	Level	Pattern	Image	Note
TS19: head mesenchyme	Present		3D , 3C	Expression is not seen in the epithelial cells of the head.

Structure	Level	Pattern	Image	Note
TS18: apical ectodermal ridge	Present		3E

Structure	Level	Pattern	Image	Note
TS18: apical ectodermal ridge	Present		3F
TS18: limb mesenchyme	Absent		3F

Structure	Level	Pattern	Image	Note
TS19: apical ectodermal ridge	Present		3G
TS19: limb mesenchyme	Present		3G

Structure	Level	Pattern	Image	Note
TS20: rib pre-cartilage condensation	Present		3H	Expression is seen in the mesenchymal cells in the thorax area in the region of cartilaginous primordia of the developing ribs.