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Gene Expression Data
Assay Details
Assay
Reference: J:81327 Downs KM, et al., Multiple developmental roles of Ahnak are suggested by localization to sites of placentation and neural plate fusion in the mouse conceptus. Mech Dev. 2002 Dec;119 Suppl 1:S31-8
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5603121
Gene symbol: Ahnak
Gene name: AHNAK nucleoprotein
Antibody: KIS antibody
Detection system: Secondary antibody
Results
Specimen 1A: embryonic day 7.5 (more )
Note: Neural plate/late allantoic bud stage.
Structure Level Pattern Image Note
TS11: allantois Present Regionally restricted 1A Expression is in the outer cells of the allantois and some distal allantoic core cells.
TS11: amnion mesoderm Present 1A
TS11: blood island Present 1A
TS11: yolk sac mesoderm Present 1A

Specimen 1B: embryonic day 8.0 (more )
Note: 2 somites.
Structure Level Pattern Image Note
TS12: allantois Present Regionally restricted 1B Expression is in the outer cells of the allantois and some distal and basal allantoic core cells.
TS12: chorion ectoderm Present 1B
TS12: chorion mesenchyme Present 1B

Specimen 1C: embryonic day 8.5 (more )
Note: 7 somites.
Structure Level Pattern Image Note
TS13: chorion Present Regionally restricted 1C Expression is in the mesothelium.

Specimen 1D: embryonic day 9.5 (more )
Note: 18 somites.
Structure Level Pattern Image Note
TS15: extraembryonic blood vessel Present Regionally restricted 1D Expression is in the endothelium of the nascent allantoic vasculature.
TS15: allantois Present Regionally restricted 1D Expression is in allantoic core cells.
TS15: extraembryonic component Present Regionally restricted 1D Expression is in mesothelium.

Specimen 1E: embryonic day 9.5 (more )
Note: 18 somites.
Structure Level Pattern Image Note
TS15: chorioallantoic placenta Strong Regionally restricted 1E Expression is in peripheral intermingled chorio-allantoic mesoderm.
TS15: allantois Strong Regionally restricted 1E Expression is at the periphery of the chorio-allantoic fusion junction, where adhesion between allantoic and chorionic mesothelial cells secures the umbilicus onto the chorionic disk.
TS15: chorionic plate Strong Regionally restricted 1E Expression is at the periphery of the chorio-allantoic fusion junction, where adhesion between allantoic and chorionic mesothelial cells secures the umbilicus onto the chorionic disk.

Specimen 1G: embryonic day 9.5 (more )
Note: 27 somites. Double labeled: brown - Ahnak; blue - Kdr (assay MGI:5603122).
Structure Level Pattern Image Note
TS15: trophectoderm Present Regionally restricted 1G Expression is in syncytiotrophoblasts and in some trophoblast derivatives of the basal zone.

Specimen 1H: embryonic day 9.5 (more )
Note: 27 somites. Double labeled: brown - Ahnak; blue - Kdr (assay MGI:5603122).
Structure Level Pattern Image Note
TS15: trophectoderm Present Regionally restricted 1H Expression is in syncytiotrophoblasts and in some trophoblast derivatives of the basal zone.

Specimen 1I: embryonic day 9.5 (more )
Note: 27 somites. Double labeled: brown - Ahnak; blue - Kdr (assay MGI:5603122).
Structure Level Pattern Image Note
TS15: trophectoderm Present Regionally restricted 1I Expression is in syncytiotrophoblasts.

Specimen 2A: embryonic day 7.5 (more )
Note: Neural plate/late allantoic bud stage.
Structure Level Pattern Image Note
TS11: chorion ectoderm Present Regionally restricted 2A
TS11: chorion mesoderm Present Regionally restricted 2A
TS11: ectoplacental cone Weak 2A
TS11: primary trophoblast giant cell Weak Single cells 2A

Specimen 2B: embryonic day 8.25 (more )
Note: 4 somites.
Structure Level Pattern Image Note
TS13: chorion Present Regionally restricted 2B Expression is in chorionic mesoderm and in the cytoplasm of peripheral chorionic ectoderm cells.

Specimen 2C: embryonic day 8.25 (more )
Note: 4 somites.
Structure Level Pattern Image Note
TS13: chorion Present Regionally restricted 2C Expression is in the chorionic mesoderm and the apical edge of peripheral chorionic ectoderm cells.

Specimen 2D: embryonic day 8.25 (more )
Note: 5 somites.
Structure Level Pattern Image Note
TS13: chorion Present Regionally restricted 2D Expression is in chorionic ectoderm.

Specimen 2E: embryonic day 7.5 (more )
Note: Neural plate/late allantoic bud stage.
Structure Level Pattern Image Note
TS11: trophectoderm Present Regionally restricted 2E There is peripheral staining in secondary trophoblast giant cells.

Specimen 2F: embryonic day 8.25 (more )
Note: 4 somites.
Structure Level Pattern Image Note
TS13: extraembryonic component Present Single cells 2H , 2F Expression is in some parietal endoderm cells.
TS13: trophoblast giant cell Present 2H , 2F

Specimen 2F mother: postnatal adult (more )
Note: H and I are higher magnifications of the decidua capsularis in F. J is a higher magnification of the decidua vascularis in F. K is a higher magnification of the decidua basalis in F.
Structure Level Pattern Image Note
TS28: decidua capsularis Present 2F , 2H , 2I
TS28: decidua basalis Weak 2F , 2K
TS28: decidua Present Regionally restricted 2J , 2F Expression is in the decidua vascularis.

Specimen not shown: embryonic day 8.25 (more )
Note: 7 somites.
Structure Level Pattern Image Note
TS13: hindgut diverticulum endoderm Present

Specimen 3A: embryonic day 7.5 (more )
Note: Neural plate/late allantoic bud stage.
Structure Level Pattern Image Note
TS11: embryo mesoderm Present Regionally restricted 3A Expression is in the anterior mesoderm.
TS11: node Present 3A
TS11: primitive endoderm Present 3A
TS11: neural ectoderm Absent 3A

Specimen 3B: embryonic day 8.0 (more )
Note: Early headfold stage.
Structure Level Pattern Image Note
TS12: head mesenchyme Present 3B
TS12: heart Present 3B
TS12: neural ectoderm Absent 3B
TS12: primitive streak Absent 3B
TS12: somite Absent 3B

Specimen 3C: embryonic day 8.25 (more )
Note: 3 somites.
Structure Level Pattern Image Note
TS13: embryo endoderm Present 3C
TS13: heart Present 3C
TS13: mesenchyme derived from head mesoderm Present 3C
TS13: embryo Present Regionally restricted 3C Expression is absent in the posterior region.
TS13: neural ectoderm Absent 3C
TS13: somite Absent 3C

Specimen 3D: embryonic day 8.25 (more )
Note: 5 somites.
Structure Level Pattern Image Note
TS13: head mesenchyme Present 3D
TS13: heart Present 3D

Specimen 3E: embryonic day 8.25 (more )
Note: 5 somites.
Structure Level Pattern Image Note
TS13: trunk Present Regionally restricted 3E Expression is in the endodermal component of the trunk.
TS13: neural ectoderm Present Regionally restricted 3E Expression is at the posterior surface of the trunk in surface ectoderm and/or neurectoderm.
TS13: surface ectoderm Present Regionally restricted 3E Expression is at the posterior surface of the trunk in surface ectoderm and/or neurectoderm.
TS13: primitive streak Absent 3E
TS13: somite Absent 3E

Specimen 3F: embryonic day 8.5 (more )
Note: 7 somites.
Structure Level Pattern Image Note
TS13: foregut diverticulum endoderm Present 3F
TS13: head mesenchyme Present 3F
TS13: heart Present 3F
TS13: surface ectoderm Present Regionally restricted 3F Expression is in the surface ectoderm overlying the hindbrain.

Specimen 3G: embryonic day 9.0 (more )
Note: 16 somites.
Structure Level Pattern Image Note
TS14: future midbrain Present Regionally restricted 3G Expression is in the dorsal tips prior to cranial fold closure.

Specimen 3H: embryonic day 9.0 (more )
Note: 16 somites.
Structure Level Pattern Image Note
TS14: future midbrain Present Regionally restricted 3H Expression is in the dorsal tips during closure.

Specimen 3I: embryonic day 9.0 (more )
Note: 16 somites.
Structure Level Pattern Image Note
TS14: future midbrain Present Regionally restricted 3I Expression is in the dorsal tips during closure.

Specimen 3J: embryonic day 9.0 (more )
Note: 16 somites.
Structure Level Pattern Image Note
TS14: future midbrain Present Regionally restricted 3J Expression is in the dorsal tips after closure.

Specimen 4A: embryonic day 8.5-9.0 (more )
Note: 8 somites.
Structure Level Pattern Image Note
TS14: future spinal cord neural plate Present Regionally restricted 4A Expression is at the peripheral edges of the flattened neural plate.
TS14: surface ectoderm Present Regionally restricted 4A Expression is in the surface ectoderm overlying the neural folds.
TS14: future spinal cord neural crest Present 4A

Specimen 4B: embryonic day 8.5-9.0 (more )
Note: 8 somites.
Structure Level Pattern Image Note
TS14: future spinal cord neural fold Present Regionally restricted 4B Expression is at the dorsal tips.
TS14: surface ectoderm Present Regionally restricted 4B Expression is in the surface ectoderm overlying the neural folds.
TS14: future spinal cord neural crest Present 4B

Specimen 4C: embryonic day 8.5-9.0 (more )
Note: 8 somites.
Structure Level Pattern Image Note
TS14: future spinal cord neural fold Very strong Regionally restricted 4C Expression is at the dorsal tips.

Specimen 4D: embryonic day 8.5-9.0 (more )
Note: 8 somites.
Structure Level Pattern Image Note
TS14: neural tube Very strong Regionally restricted 4D Expression is dorsal.
TS14: surface ectoderm Present 4D

Specimen 4E: embryonic day 8.5-9.0 (more )
Note: 8 somites.
Structure Level Pattern Image Note
TS14: neural tube Present Regionally restricted 4E Expression is dorsal and strong at the seam of closure.
TS14: notochord Strong 4E
TS14: surface ectoderm Present 4E Expression is in the surface ectoderm overlying the neural tube.

Specimen 4F: embryonic day 9.5 (more )
Note: 19 somites.
Structure Level Pattern Image Note
TS15: neural tube Present Regionally restricted 4F Expression is at the distal edges of the flat neural plate.
TS15: surface ectoderm Present Regionally restricted 4F Expression is in the surface ectoderm overlying the neural folds.

Specimen 4G: embryonic day 9.5 (more )
Note: 19 somites.
Structure Level Pattern Image Note
TS15: neural tube Present 4G Expression is at the distal edges of the closing neural plate.
TS15: surface ectoderm Present Regionally restricted 4G Expression is in the surface ectoderm overlying the closing neural folds.

Specimen 4H: embryonic day 9.5 (more )
Note: 19 somites.
Structure Level Pattern Image Note
TS15: neural tube Present Regionally restricted 4H Expression is at the distal edges of the closing neural plate.
TS15: surface ectoderm Present Regionally restricted 4H Expression is in the surface ectoderm overlying the closing neural folds.

Specimen 4I: embryonic day 9.5 (more )
Note: 19 somites.
Structure Level Pattern Image Note
TS15: neural tube Present Regionally restricted 4I Expression is at the distal edges of the closing neural plate.
TS15: surface ectoderm Present Regionally restricted 4I Expression is in the surface ectoderm overlying the neural tube.

Specimen 4J: embryonic day 9.5 (more )
Note: 19 somites.
Structure Level Pattern Image Note
TS15: neural tube Present Regionally restricted 4J Expression is dorsal.
TS15: surface ectoderm Present Regionally restricted 4J Expression is in the surface ectoderm overlying the neural tube.

Specimen 4K: embryonic day 10.0 (more )
Note: 27 somites.
Structure Level Pattern Image Note
TS16: neural tube Present Regionally restricted 4K Expression is at the dorsal edges of the closing neural plate.
TS16: surface ectoderm Present Regionally restricted 4K Expression is in the surface ectoderm overlying the neural folds.

Specimen 4L: embryonic day 10.0 (more )
Note: 27 somites.
Structure Level Pattern Image Note
TS16: neural tube Present Regionally restricted 4L Expression is at the dorsal edges of the closing neural plate.
TS16: surface ectoderm Present Regionally restricted 4L Expression is in the surface ectoderm overlying the closing neural folds.

Specimen 4M: embryonic day 10.0 (more )
Note: 27 somites.
Structure Level Pattern Image Note
TS16: neural tube Present Regionally restricted 4M Expression is at the dorsal edges of the closing neural plate.
TS16: surface ectoderm Present Regionally restricted 4M Expression is in the surface ectoderm overlying the closing neural folds.

Specimen 4N: embryonic day 10.0 (more )
Note: 27 somites.
Structure Level Pattern Image Note
TS16: neural tube Present Regionally restricted 4N Expression is at the dorsal edges.
TS16: surface ectoderm Present Regionally restricted 4N Expression is in the surface ectoderm overlying the neural tube.

Specimen 4O: embryonic day 10.0 (more )
Note: 27 somites.
Structure Level Pattern Image Note
TS16: neural tube Present Regionally restricted 4O Expression is at the dorsal edges.
TS16: surface ectoderm Present Regionally restricted 4O Expression is in the surface ectoderm overlying the neural tube.

Specimen 5A: embryonic day 10.0 (more )
Note: 31 somites.
Structure Level Pattern Image Note
TS16: tail future spinal cord Present Regionally restricted 5A Expression is in the dorso-medial region of the closed neural tube, absent during secondary neurulation (shown).

Specimen 5B: embryonic day 10.0 (more )
Note: 31 somites.
Structure Level Pattern Image Note
TS16: tail future spinal cord Present Regionally restricted 5B Expression is in the dorso-medial region of the closed neural tube, absent during secondary neurulation (shown).

Specimen 5C: embryonic day 10.0 (more )
Note: 31 somites.
Structure Level Pattern Image Note
TS16: tail future spinal cord Present Regionally restricted 5C Expression is in the dorso-medial region of the closed neural tube, absent during secondary neurulation (shown).

Specimen 5D: embryonic day 10.0 (more )
Note: 31 somites.
Structure Level Pattern Image Note
TS16: tail future spinal cord Present Regionally restricted 5D Expression is in the dorso-medial region of the closed neural tube, absent during secondary neurulation (shown).

Specimen 5E: embryonic day 10.0 (more )
Note: 31 somites.
Structure Level Pattern Image Note
TS16: tail future spinal cord Present Regionally restricted 5E Expression is in the dorso-medial region.

Specimen 5F: embryonic day 10.0 (more )
Note: 31 somites.
Structure Level Pattern Image Note
TS16: tail future spinal cord Present Regionally restricted 5F Expression is in the dorso-medial region.

Specimen 5G: embryonic day 10.0 (more )
Note: 31 somites.
Structure Level Pattern Image Note
TS16: tail future spinal cord Present Regionally restricted 5G Expression is in the dorso-medial region.

Specimen 5H: embryonic day 10.0 (more )
Note: 31 somites.
Structure Level Pattern Image Note
TS16: tail future spinal cord Present Regionally restricted 5H Expression is in the dorso-medial region.


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last database update
07/02/2024
MGI 6.13
The Jackson Laboratory