Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:212101
Kamiya K, et al., An unusually powerful mode of low-frequency sound interference due to defective hair bundles of the auditory outer hair cells. Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):9307-12
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:5602878
|
|
Gene symbol:
|
Nherf1
|
|
Gene name:
|
NHERF family PDZ scaffold protein 1
|
|
|
Results
|
Specimen
S3A wt:
postnatal day 3
(more  )
Specimen
S3A wt:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal day 3
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
Specimen
S3A -/-:
(close )
|
Genetic Background:
|
involves: C57BL/6J
|
|
Mutant Allele(s):
|
Nherf1tm1Geo/Nherf1tm1Geo
|
|
Age:
|
postnatal day 3
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
Specimen
S3B E15.5:
embryonic day 15.5
(more )
Specimen
S3B E15.5:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cochlea epithelium |
Present |
Graded |
S3B E15.5
|
Expression is present in the hair bundles of both inner hair cells (IHC) and outer hair cells (OHC). Staining is more intense in the cochlear base compared to the apex.
|
Specimen
S3B E17:
embryonic day 17.0-17.5
(more )
Specimen
S3B E17:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
embryonic day 17.0-17.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: cochlea epithelium |
Present |
Regionally restricted |
S3B E17
|
Expression is present in the hair bundles throughout the cochlea in both inner hair cells (IHC) and outer hair cells (OHC), as well as in the microvilli of surrounding supporting cells.
|
Specimen
S3B E18:
embryonic day 18.0-18.5
(more )
Specimen
S3B E18:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
embryonic day 18.0-18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cochlea epithelium |
Present |
Regionally restricted |
S3B E18
|
Expression is present in the hair bundles throughout the cochlea in both inner hair cells and outer hair cells, as well as in the microvilli of surrounding supporting cells.
|
Specimen
S3B P0:
postnatal day 0
(more )
Specimen
S3B P0:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: organ of Corti |
Present |
Regionally restricted |
S3B P0
|
The protein is no longer detected in inner hair cells but its level increased in the outer hair cell bundles relative to levels seen in the embryo.
|
Specimen
S3B P3:
postnatal day 3
(more )
Specimen
S3B P3:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
postnatal day 3
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: organ of Corti |
Present |
Regionally restricted |
S3B P3
|
The protein is no longer detected in inner hair cells but its level increased in the outer hair cell bundles relative to levels seen in the embryo.
|
Specimen
S3B P5:
postnatal day 5
(more )
Specimen
S3B P5:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
postnatal day 5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
Present |
Regionally restricted |
S3B P5
|
The protein is no longer detected in inner hair cells (IHC) but its level increased in the outer hair cell (OHC) bundles relative to levels seen in the embryo.
|
Specimen
S3B P10:
postnatal day 10
(more )
Specimen
S3B P10:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
postnatal day 10
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
Present |
Regionally restricted |
S3B P10
|
The protein is no longer detected in inner hair cells (IHC) but is present in the outer hair cell (OHC) bundles.
|
Specimen
1A E16:
embryonic day 16.0
(more )
Specimen
1A E16:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
embryonic day 16.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cochlea epithelium |
Present |
Regionally restricted |
1A E16
|
Expression is present in the hair bundles of both inner hair cells (IHC) and outer hair cells (OHC)
|
Specimen
1A P0:
postnatal day 0
(more )
Specimen
1A P0:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: organ of Corti |
Present |
Regionally restricted |
1A P0
|
Expression is present in the hair bundles of outer hair cells (OHC) but not in the inner hair cells (IHC). It is intense at the tips of the differentiating stereocilia.
|
Specimen
1A P12:
postnatal day 12
(more )
Specimen
1A P12:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
postnatal day 12
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
Present |
Regionally restricted |
1A P12
|
Expression is present in the hair bundles of outer hair cells (OHC) but not in the inner hair cells (IHC). It is intense at the tips of the differentiating stereocilia.
|
Specimen
1B:
postnatal day 5
(more )
Note: Triple labeled: green - Nherf1; blue - Cdh23 (assay MGI:5602951); red- F-actin.
Specimen
1B:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal day 5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Note:
|
Triple labeled: green - Nherf1; blue - Cdh23 (assay MGI:5602951); red- F-actin.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
Present |
Regionally restricted |
1B left
,
1B right
|
Expression is present in outer hair cell (OHC) bundles in the tips of the stereocilia.
|
Specimen
1C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Cdh23v-2J/Cdh23v-2J
|
|
Age:
|
postnatal day 5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: organ of Corti |
Present |
Regionally restricted |
1C right
,
1C left
|
In the mutant expression levels are substantially reduced in the stereocilia of hair cells, although levels are unchanged in supporting cells.
|
|
Analysis Tools