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Gene Expression Data
Assay Details
Assay
Reference: J:207639 Nelson DO, et al., Irx4 identifies a chamber-specific cell population that contributes to ventricular myocardium development. Dev Dyn. 2014 Mar;243(3):381-92
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5588432
Gene symbol: Irx4
Gene name: Iroquois homeobox 4
Antibody: Anti-Irx4 (N-16) sc-22582
Detection system: Secondary antibody coupled to Alexa Fluor 568
Assay notes: Two antibodies were described in the paper - MGI:5588406 and MGI:5588410.
Results
Specimen 3B,D,D': embryonic day 9.0 (more )
Note: Double labeled: red - Irx4; green - Nkx2-5 (assay MGI:5588433).
Structure Level Pattern Image Note
TS14: endocardial tube Present Regionally restricted 3B , 3D' , 3D Expression was restricted to the ventricular segment of the linear heart tube and colocalized with Nkx2-5. Expression was not detected at the arterial or venous poles of the heart tube.
Specimen 4B,D,D': embryonic day 10.5 (more )
Note: Double labeled: red - Irx4; green - Tnnt2 (assay MGI:5588663).
Structure Level Pattern Image Note
TS17: primitive ventricle cardiac muscle Present Regionally restricted 4D' , 4B , 4D Expression was detected in the compact zone of the ventricular myocardium. Co-expression with cardiac troponin T was noted.
Specimen 4F,H,H': embryonic day 12.5 (more )
Note: Double labeled: red - Irx4; green - Tnnt2 (assay MGI:5588663).
Structure Level Pattern Image Note
TS20: ventricle cardiac muscle Present Regionally restricted 4H' , 4H , 4F Expression was detected in the compact zone with diminished expression in the trabecular region. Co-expression with cardiac troponin T was noted.
Specimen 4J,L,L': embryonic day 14.5 (more )
Note: Double labeled: red - Irx4; green - Tnnt2 (assay MGI:5588663).
Structure Level Pattern Image Note
TS22: ventricle cardiac muscle Present Regionally restricted 4J , 4L , 4L' Expression was detected in the compact zone with diminished expression in the trabecular region. Co-expression with cardiac troponin T was noted.
Specimen 5B,D: postnatal day 2 (more )
Note: Double labeled: red - Irx4; green - Tnnt2 (assay MGI:5588663).
Structure Level Pattern Image Note
TS27: ventricle cardiac muscle Present 5D , 5B Expression was restricted to the cytoplasm.
Specimen 5F,H: postnatal day 4 (more )
Note: Double labeled: red - Irx4; green - Tnnt2 (assay MGI:5588663).
Structure Level Pattern Image Note
TS28: ventricle cardiac muscle Present 5H , 5F Expression was restricted to the cytoplasm.
Specimen 5J,L: postnatal day 6 (more )
Note: Double labeled: red - Irx4; green - Tnnt2 (assay MGI:5588663).
Structure Level Pattern Image Note
TS28: ventricle cardiac muscle Present 5L , 5J Expression translocates to the nucleus between P5-7.
Specimen 5N,P: postnatal day 8 (more )
Note: Double labeled: red - Irx4; green - Tnnt2 (assay MGI:5588663).
Structure Level Pattern Image Note
TS28: ventricle cardiac muscle Present 5N , 5P Expression was restricted to the nucleus.
Specimen 5R,T: postnatal day 10 (more )
Note: Double labeled: red - Irx4; green - Tnnt2 (assay MGI:5588663).
Structure Level Pattern Image Note
TS28: ventricle cardiac muscle Present 5T , 5R Expression was restricted to the nucleus.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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