Antibody:	clone 39.4D5
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS17: embryo endoderm	Present		1d
TS17: heart mesentery	Present	Regionally restricted	1d	Expressed in the anterior second heart field mesoderm and faded towards the differentiating myocardium of the outflow tract. Expressed as a central cluster of cells ventral to the foregut, on the left side of the aortic sac. Absent on the right side.
TS17: outflow tract cardiac muscle	Present	Regionally restricted	1d	Expressed in some of the myocardial cells.
TS17: visceral pericardium	Present	Regionally restricted	1d	Expressed in the coelomic wall lining, being also more prominent on the left side as compared to the right side.

Structure	Level	Pattern	Image	Note
TS17: pharynx mesenchyme	Present	Regionally restricted	4c	Expressed as a group of positive cells located behind the heart in the pre-pharyngeal mesoderm.
TS17: outflow tract cardiac muscle	Present	Regionally restricted	4c	Expressed as an elongated column extending along the left side.
TS17: heart mesentery	Present	Regionally restricted	4c	Expressed as a well-organized cluster of cells in the cardiac mesenchyme.

Structure	Level	Pattern	Image	Note
TS17: outflow tract cardiac muscle	Present	Regionally restricted	4f	Expressed in an abnormal very short column of cells extending along the left side.

Structure	Level	Pattern	Image	Note
TS19: pharynx mesenchyme	Present	Regionally restricted		Expressed as a central cluster of cells located in the pre-pharyngeal mesoderm dorsal to the level of the outflow tract.
TS19: outflow tract	Present	Regionally restricted		Expression in the cardiac precursor cells showed an asymmetric distribution favouring the pulmonary side. No expression in the myocardium.