Gene Expression Data
Assay Details
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Assay
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Reference:
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J:166980
Ishikawa T, et al., IgSF molecule MDGA1 is involved in radial migration and positioning of a subset of cortical upper-layer neurons. Dev Dyn. 2011 Jan;240(1):96-107
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5584400
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Gene symbol:
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Mdga1
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Gene name:
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MAM domain containing glycosylphosphatidylinositol anchor 1
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Reporter:
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lacZ
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Probe:
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lacZ probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1D right:
(close  )
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Genetic Background:
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B6.Cg-Mdga1tm1.1Tymo
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Mutant Allele(s):
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Mdga1tm1.1Tymo/Mdga1+
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Age:
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postnatal day 14
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain |
Present |
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1D right
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Expression matched the endogenous gene expression.
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Specimen
2B Motor LacZ/LacZ:
(close )
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Genetic Background:
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B6.Cg-Mdga1tm1.1Tymo
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Mutant Allele(s):
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Mdga1tm1.1Tymo/Mdga1tm1.1Tymo
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Age:
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postnatal day 14
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2B Sensory LacZ/LacZ:
(close )
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Genetic Background:
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B6.Cg-Mdga1tm1.1Tymo
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Mutant Allele(s):
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Mdga1tm1.1Tymo/Mdga1tm1.1Tymo
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Age:
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postnatal day 14
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2B Visual LacZ/LacZ:
(close )
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Genetic Background:
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B6.Cg-Mdga1tm1.1Tymo
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Mutant Allele(s):
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Mdga1tm1.1Tymo/Mdga1tm1.1Tymo
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Age:
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postnatal day 14
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3 rostral -/-:
(close )
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Genetic Background:
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B6.Cg-Mdga1tm1.1Tymo
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Mutant Allele(s):
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Mdga1tm1.1Tymo/Mdga1tm1.1Tymo
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Age:
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postnatal day 14
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3 medial -/-:
(close )
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Genetic Background:
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B6.Cg-Mdga1tm1.1Tymo
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Mutant Allele(s):
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Mdga1tm1.1Tymo/Mdga1tm1.1Tymo
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Age:
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postnatal day 14
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3 caudal -/-:
(close )
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Genetic Background:
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B6.Cg-Mdga1tm1.1Tymo
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Mutant Allele(s):
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Mdga1tm1.1Tymo/Mdga1tm1.1Tymo
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Age:
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postnatal day 14
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4 LacZ/LacZ:
(close )
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Genetic Background:
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B6.Cg-Mdga1tm1.1Tymo
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Mutant Allele(s):
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Mdga1tm1.1Tymo/Mdga1tm1.1Tymo
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cortical plate |
Present |
Regionally restricted |
4_LacZ/LacZ LacZ
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The majority of the positive cells resided in the deeper region of the cortical plate, beneath future layer V neurons.
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Specimen
5 LacZ/LacZ:
(close )
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Genetic Background:
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B6.Cg-Mdga1tm1.1Tymo
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Mutant Allele(s):
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Mdga1tm1.1Tymo/Mdga1tm1.1Tymo
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: cortical plate |
Present |
Regionally restricted |
5_LacZ/LacZ LacZ
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Some of the positive cells had migrated to the upper region, most remained beneath the future layer V neurons.
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Specimen
6 LacZ/LacZ:
(close )
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Genetic Background:
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B6.Cg-Mdga1tm1.1Tymo
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Mutant Allele(s):
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Mdga1tm1.1Tymo/Mdga1tm1.1Tymo
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cortical plate |
Present |
Regionally restricted |
6_LacZ/LacZ LacZ
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Majority of the positive cells had migrated above the layer V neurons, still located in a relatively deeper layer compared with the wild-type.
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Specimen
7 LacZ/LacZ:
(close )
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Genetic Background:
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B6.Cg-Mdga1tm1.1Tymo
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Mutant Allele(s):
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Mdga1tm1.1Tymo/Mdga1tm1.1Tymo
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: neocortex layer |
Present |
Regionally restricted |
7_LacZ/LacZ LacZ
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Majority of the positive cells had migrated above the layer V neurons, mostly located in a deeper layer compared with the wild-type. Some positive cells were beneath Layer V.
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Specimen
8 LacZ/LacZ:
(close )
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Genetic Background:
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B6.Cg-Mdga1tm1.1Tymo
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Mutant Allele(s):
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Mdga1tm1.1Tymo/Mdga1tm1.1Tymo
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: neocortex layer |
Present |
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8_LacZ/LacZ LacZ
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Expression was indistinguishable from the positions of the Mdga1-expressing cells in their wild-type littermates.
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Analysis Tools
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